Production of l-theanine using glutaminase encapsulated in carbon-coated mesoporous silica with high pH stability

Tetsuji Itoh, Yasuto Hoshikawa, Shun Ichi Matsuura, Junko Mizuguchi, Hiroyuki Arafune, Taka Aki Hanaoka, Fujio Mizukami, Akari Hayashi, Hirotomo Nishihara, Takashi Kyotani

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

A new enzymatic process for production of l-theanine was presented using glutaminase combined with immobilization technique on carbon-coated mesoporous silica (carbon/SBA-15). For the attempt to enhance the durability against high pH conditions, the pore surface of mesoporous silica SBA-15 was coated with a thin layer of carbon. Carbon coating was done by addition of 2,3-dihydroxynaphthalene (DN) on the pore surface, followed by a dehydration reaction between surface silanol groups in SBA-15 and hydroxyl groups of DN molecules, and further carbonization of DN. A glutaminase was confined in nanospace (about 5. nm) of carbon/SBA-15. The resulting product, glutaminase/carbon/SBA-15, successfully catalyzed the reaction for production of l-theanine under high pH conditions.

Original languageEnglish
Pages (from-to)207-214
Number of pages8
JournalBiochemical Engineering Journal
Volume68
DOIs
Publication statusPublished - Oct 15 2012

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Environmental Engineering
  • Bioengineering
  • Biomedical Engineering

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