Production of monoclonal antibodies against a major purgative component, sennoside B, their characterization and use in ELISA

O. Morinaga, S. Nakajima, Hiroyuki Tanaka, Y. Shoyama

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

For immunization, sennoside B was conjugated with bovine serum albumin. The hapten density in the antigen conjugate was determined to be 3 mol mol-1 protein by matrix-assisted laser desorption-ionization TOF mass spectrometry. A hybridoma secreting monoclonal antibody against sennoside B was produced by fusing splenocytes from mouse immunized with the sennoside B conjugate and mouse myeloma cells. Weak cross-reactivities occurred with sennoside A which is a stereochemical isomer, and a monomer of sennoside B, rhein, but no cross-reactivity was observed with other related anthraquinones and phenolics. The range of the assay extended from 0.5 ng ml-1 to 15 ng ml-1 of sennoside B, and good correlation between ELISA and HPLC methods was obtained when crude extracts of rhubarb were analyzed.

Original languageEnglish
Pages (from-to)1372-1376
Number of pages5
JournalAnalyst
Volume126
Issue number8
DOIs
Publication statusPublished - Aug 25 2001

Fingerprint

Cathartics
Immunization
Monoclonal antibodies
immunization
Antigens
antigen
Isomers
antibody
Ionization
Mass spectrometry
serum
Assays
Desorption
desorption
ionization
mass spectrometry
Monomers
laser
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

Cite this

Production of monoclonal antibodies against a major purgative component, sennoside B, their characterization and use in ELISA. / Morinaga, O.; Nakajima, S.; Tanaka, Hiroyuki; Shoyama, Y.

In: Analyst, Vol. 126, No. 8, 25.08.2001, p. 1372-1376.

Research output: Contribution to journalArticle

Morinaga, O. ; Nakajima, S. ; Tanaka, Hiroyuki ; Shoyama, Y. / Production of monoclonal antibodies against a major purgative component, sennoside B, their characterization and use in ELISA. In: Analyst. 2001 ; Vol. 126, No. 8. pp. 1372-1376.
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