Production of monoclonal antibodies against a major purgative component, sennoside B, their characterization and use in ELISA

O. Morinaga, S. Nakajima, H. Tanaka, Y. Shoyama

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

For immunization, sennoside B was conjugated with bovine serum albumin. The hapten density in the antigen conjugate was determined to be 3 mol mol-1 protein by matrix-assisted laser desorption-ionization TOF mass spectrometry. A hybridoma secreting monoclonal antibody against sennoside B was produced by fusing splenocytes from mouse immunized with the sennoside B conjugate and mouse myeloma cells. Weak cross-reactivities occurred with sennoside A which is a stereochemical isomer, and a monomer of sennoside B, rhein, but no cross-reactivity was observed with other related anthraquinones and phenolics. The range of the assay extended from 0.5 ng ml-1 to 15 ng ml-1 of sennoside B, and good correlation between ELISA and HPLC methods was obtained when crude extracts of rhubarb were analyzed.

Original languageEnglish
Pages (from-to)1372-1376
Number of pages5
JournalAnalyst
Volume126
Issue number8
DOIs
Publication statusPublished - Jan 1 2001

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

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