Production of Monoclonal Antibodies Against Leptospiral Genus-Specific Protein Antigen and Localization of the Antigen by Immunoelectron Microscopy

Noritsugu Sakamoto; Etsuro Ono; Hiroshi Kida; Ryo Yanagawa

doi:10.1016/S0176-6724(85)80088-2

Production of Monoclonal Antibodies Against Leptospiral Genus-Specific Protein Antigen and Localization of the Antigen by Immunoelectron Microscopy

Noritsugu Sakamoto, Etsuro Ono, Hiroshi Kida, Ryo Yanagawa

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Monoclonal antibodies were first produced against a leptospiral genus-specific protein antigen (GP-Ag) isolated from Leptospira interrogans serovar kremastos strain Kyoto. The representative monoclonal antibody (GP-7) did not agglutinate kremastos Kyoto but reacted with Triton X-100 treated kreamastos Kyoto, andamana, patoc and Urawa in enzyme-linked immunosorbent assay (ELISA), indicating that the monoclonal antibody recognized the genus-specific determinant of GP-Ag. The localization of GP-Ag was investigated by immunoelectron microscopy with the iodinated antibody and peroxidase-labeled antibody. The genus-specific determinant of GP-Ag recognized by GP-7 was located on the subsurface of the leptospiral envelope.

Original language	English
Pages (from-to)	557-563
Number of pages	7
Journal	Zentralblatt fur Bakteriologie Mikrobiologie und Hygiene - Abt. 1 Orig. A
Volume	259
Issue number	4
DOIs	https://doi.org/10.1016/S0176-6724(85)80088-2
Publication status	Published - 1985
Externally published	Yes

All Science Journal Classification (ASJC) codes

Immunology

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10.1016/S0176-6724(85)80088-2

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Production of Monoclonal Antibodies Against Leptospiral Genus-Specific Protein Antigen and Localization of the Antigen by Immunoelectron Microscopy. / Sakamoto, Noritsugu; Ono, Etsuro; Kida, Hiroshi et al.
In: Zentralblatt fur Bakteriologie Mikrobiologie und Hygiene - Abt. 1 Orig. A, Vol. 259, No. 4, 1985, p. 557-563.

Research output: Contribution to journal › Article › peer-review

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title = "Production of Monoclonal Antibodies Against Leptospiral Genus-Specific Protein Antigen and Localization of the Antigen by Immunoelectron Microscopy",

abstract = "Monoclonal antibodies were first produced against a leptospiral genus-specific protein antigen (GP-Ag) isolated from Leptospira interrogans serovar kremastos strain Kyoto. The representative monoclonal antibody (GP-7) did not agglutinate kremastos Kyoto but reacted with Triton X-100 treated kreamastos Kyoto, andamana, patoc and Urawa in enzyme-linked immunosorbent assay (ELISA), indicating that the monoclonal antibody recognized the genus-specific determinant of GP-Ag. The localization of GP-Ag was investigated by immunoelectron microscopy with the iodinated antibody and peroxidase-labeled antibody. The genus-specific determinant of GP-Ag recognized by GP-7 was located on the subsurface of the leptospiral envelope.",

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AU - Sakamoto, Noritsugu

AU - Ono, Etsuro

AU - Kida, Hiroshi

AU - Yanagawa, Ryo

PY - 1985

Y1 - 1985

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AB - Monoclonal antibodies were first produced against a leptospiral genus-specific protein antigen (GP-Ag) isolated from Leptospira interrogans serovar kremastos strain Kyoto. The representative monoclonal antibody (GP-7) did not agglutinate kremastos Kyoto but reacted with Triton X-100 treated kreamastos Kyoto, andamana, patoc and Urawa in enzyme-linked immunosorbent assay (ELISA), indicating that the monoclonal antibody recognized the genus-specific determinant of GP-Ag. The localization of GP-Ag was investigated by immunoelectron microscopy with the iodinated antibody and peroxidase-labeled antibody. The genus-specific determinant of GP-Ag recognized by GP-7 was located on the subsurface of the leptospiral envelope.

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Production of Monoclonal Antibodies Against Leptospiral Genus-Specific Protein Antigen and Localization of the Antigen by Immunoelectron Microscopy

Abstract

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