Production of Monoclonal Antibodies Against Leptospiral Genus-Specific Protein Antigen and Localization of the Antigen by Immunoelectron Microscopy

Noritsugu Sakamoto, Etsuro Ono, Hiroshi Kida, Ryo Yanagawa

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10 Citations (Scopus)

Abstract

Monoclonal antibodies were first produced against a leptospiral genus-specific protein antigen (GP-Ag) isolated from Leptospira interrogans serovar kremastos strain Kyoto. The representative monoclonal antibody (GP-7) did not agglutinate kremastos Kyoto but reacted with Triton X-100 treated kreamastos Kyoto, andamana, patoc and Urawa in enzyme-linked immunosorbent assay (ELISA), indicating that the monoclonal antibody recognized the genus-specific determinant of GP-Ag. The localization of GP-Ag was investigated by immunoelectron microscopy with the iodinated antibody and peroxidase-labeled antibody. The genus-specific determinant of GP-Ag recognized by GP-7 was located on the subsurface of the leptospiral envelope.

Original languageEnglish
Pages (from-to)557-563
Number of pages7
JournalZentralblatt fur Bakteriologie Mikrobiologie und Hygiene - Abt. 1 Orig. A
Volume259
Issue number4
DOIs
Publication statusPublished - Jan 1 1985
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Immunology

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