Production of monoclonal antibodies specific to major allergens of Cryptomeria japonica pollen

K. Honda, H. Shirae, K. Miwa, S. Matsushita, K. Makishima, Y. Nishimura, Takehiko Sasazuki

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3 Citations (Scopus)

Abstract

Monoclonal antibodies (MAbs) directed against Cryptomeria japonica pollen antigen (CPAg) were prepared. CPAg was precipitated with these MAbs and both MAbs CPA7 (IgG(2a)) and CPA9 (IgG1) recognized two major glycoproteins (m=41kDa and 46 kDa) of CPAg. MAb CPA7 or CPA9 was coupled to CNBr activated sepharose and affinity purification of the major allergen of CPAg from the crude extract was performed. The affinity purified CPAg bound IgE antibody present in patients with Cedar pollinosis.

Original languageEnglish
Pages (from-to)361-366
Number of pages6
JournalHybridoma
Volume11
Issue number3
DOIs
Publication statusPublished - Jan 1 1992

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All Science Journal Classification (ASJC) codes

  • Immunology
  • Genetics

Cite this

Honda, K., Shirae, H., Miwa, K., Matsushita, S., Makishima, K., Nishimura, Y., & Sasazuki, T. (1992). Production of monoclonal antibodies specific to major allergens of Cryptomeria japonica pollen. Hybridoma, 11(3), 361-366. https://doi.org/10.1089/hyb.1992.11.361