Production of monoclonal antibody against ginkgolic acids in Ginkgo biloba Linn

P. Loungratana, H. Tanaka, Y. Shoyama

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

A competitive enzyme-linked immunosorbent assay (ELISA) for ginkgolic acids (GAs) was developed using monoclonal antibody (MAb) 9F raised against 6-(13-formylheptyl) salicylic acid covalently coupled to bovine serum albumin (BSA). ELISA, at an effective measuring range of 300 ng/ml-1 μg/ml of GA15:1. was successful in detecting GAs content in ginkgo leaves and standardized extracts due to the lack of cross-reactivity against various related compounds. The sensitive and simple immunoassay developed in this study was validated to be specific for the quantitative determination of total GAs content in ginkgo crude drugs with no interference from the sample matrix. The analytical recovery of spiked GA15:1 was 103% in a concentration range between 10 and 40 mg/g dry weight of ginkgo leaves.

Original languageEnglish
Pages (from-to)33-48
Number of pages16
JournalAmerican Journal of Chinese Medicine
Volume32
Issue number1
DOIs
Publication statusPublished - Aug 4 2004

All Science Journal Classification (ASJC) codes

  • Complementary and alternative medicine

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