TY - JOUR
T1 - Production of monoclonal antibody against ginkgolic acids in Ginkgo biloba Linn
AU - Loungratana, P.
AU - Tanaka, H.
AU - Shoyama, Y.
N1 - Funding Information:
This research was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan, the research fund from Kyushu University Foundation, and the research fund from Japan Kampo Medicine Manufacturers.
PY - 2004
Y1 - 2004
N2 - A competitive enzyme-linked immunosorbent assay (ELISA) for ginkgolic acids (GAs) was developed using monoclonal antibody (MAb) 9F raised against 6-(13-formylheptyl) salicylic acid covalently coupled to bovine serum albumin (BSA). ELISA, at an effective measuring range of 300 ng/ml-1 μg/ml of GA15:1. was successful in detecting GAs content in ginkgo leaves and standardized extracts due to the lack of cross-reactivity against various related compounds. The sensitive and simple immunoassay developed in this study was validated to be specific for the quantitative determination of total GAs content in ginkgo crude drugs with no interference from the sample matrix. The analytical recovery of spiked GA15:1 was 103% in a concentration range between 10 and 40 mg/g dry weight of ginkgo leaves.
AB - A competitive enzyme-linked immunosorbent assay (ELISA) for ginkgolic acids (GAs) was developed using monoclonal antibody (MAb) 9F raised against 6-(13-formylheptyl) salicylic acid covalently coupled to bovine serum albumin (BSA). ELISA, at an effective measuring range of 300 ng/ml-1 μg/ml of GA15:1. was successful in detecting GAs content in ginkgo leaves and standardized extracts due to the lack of cross-reactivity against various related compounds. The sensitive and simple immunoassay developed in this study was validated to be specific for the quantitative determination of total GAs content in ginkgo crude drugs with no interference from the sample matrix. The analytical recovery of spiked GA15:1 was 103% in a concentration range between 10 and 40 mg/g dry weight of ginkgo leaves.
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U2 - 10.1142/S0192415X04001709
DO - 10.1142/S0192415X04001709
M3 - Article
C2 - 15154283
AN - SCOPUS:3042536412
SN - 0192-415X
VL - 32
SP - 33
EP - 48
JO - Comparative Medicine East and West
JF - Comparative Medicine East and West
IS - 1
ER -