Production of new restriction endonuclease Vpa K111BI recognizing sequence 5’-GGWCC-3’ in a haemolysin-less mutant of vibrio parahaemolyticus

Harumi Ueno; Akihiko Kita; Michiko Miyahara; Naoko Ishiwata; Katsutoshi Mise; Ikunoshin Kato; Yoshizumi Ishino

doi:10.1271/bbb.61.2129

Production of new restriction endonuclease Vpa K111BI recognizing sequence 5’-GGWCC-3’ in a haemolysin-less mutant of vibrio parahaemolyticus

Harumi Ueno, Akihiko Kita, Michiko Miyahara, Naoko Ishiwata, Katsutoshi Mise, Ikunoshin Kato, Yoshizumi Ishino

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

High restriction endonuclease activity was found in a haemolysin-less mutant of the Vibrio parahaemolyticus 1743-1 strain. The endonuclease, named VpaK11BI, recognized the palindromic pentanucleotide sequence of 5′-GGWCC-3′ and cleaved double-stranded DNA after the first G, which is exactly the same as the specificity of AvaII. The haemolysin-less mutant of V. parahaemolyticus is now available for producing the valuable restriction endonuclease on a commercial scale.

Original language	English
Pages (from-to)	2129-2130
Number of pages	2
Journal	Bioscience, Biotechnology and Biochemistry
Volume	61
Issue number	12
DOIs	https://doi.org/10.1271/bbb.61.2129
Publication status	Published - Jan 1997
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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title = "Production of new restriction endonuclease Vpa K111BI recognizing sequence 5{\textquoteright}-GGWCC-3{\textquoteright} in a haemolysin-less mutant of vibrio parahaemolyticus",

abstract = "High restriction endonuclease activity was found in a haemolysin-less mutant of the Vibrio parahaemolyticus 1743-1 strain. The endonuclease, named VpaK11BI, recognized the palindromic pentanucleotide sequence of 5′-GGWCC-3′ and cleaved double-stranded DNA after the first G, which is exactly the same as the specificity of AvaII. The haemolysin-less mutant of V. parahaemolyticus is now available for producing the valuable restriction endonuclease on a commercial scale.",

author = "Harumi Ueno and Akihiko Kita and Michiko Miyahara and Naoko Ishiwata and Katsutoshi Mise and Ikunoshin Kato and Yoshizumi Ishino",

year = "1997",

month = jan,

doi = "10.1271/bbb.61.2129",

language = "English",

volume = "61",

pages = "2129--2130",

journal = "Bioscience, Biotechnology and Biochemistry",

issn = "0916-8451",

publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",

number = "12",

}

TY - JOUR

T1 - Production of new restriction endonuclease Vpa K111BI recognizing sequence 5’-GGWCC-3’ in a haemolysin-less mutant of vibrio parahaemolyticus

AU - Ueno, Harumi

AU - Kita, Akihiko

AU - Miyahara, Michiko

AU - Ishiwata, Naoko

AU - Mise, Katsutoshi

AU - Kato, Ikunoshin

AU - Ishino, Yoshizumi

PY - 1997/1

Y1 - 1997/1

N2 - High restriction endonuclease activity was found in a haemolysin-less mutant of the Vibrio parahaemolyticus 1743-1 strain. The endonuclease, named VpaK11BI, recognized the palindromic pentanucleotide sequence of 5′-GGWCC-3′ and cleaved double-stranded DNA after the first G, which is exactly the same as the specificity of AvaII. The haemolysin-less mutant of V. parahaemolyticus is now available for producing the valuable restriction endonuclease on a commercial scale.

AB - High restriction endonuclease activity was found in a haemolysin-less mutant of the Vibrio parahaemolyticus 1743-1 strain. The endonuclease, named VpaK11BI, recognized the palindromic pentanucleotide sequence of 5′-GGWCC-3′ and cleaved double-stranded DNA after the first G, which is exactly the same as the specificity of AvaII. The haemolysin-less mutant of V. parahaemolyticus is now available for producing the valuable restriction endonuclease on a commercial scale.

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DO - 10.1271/bbb.61.2129

M3 - Article

AN - SCOPUS:85010555670

SN - 0916-8451

VL - 61

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EP - 2130

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

IS - 12

ER -

Production of new restriction endonuclease Vpa K111BI recognizing sequence 5’-GGWCC-3’ in a haemolysin-less mutant of vibrio parahaemolyticus

Abstract

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