Production of new restriction endonuclease Vpa K111BI recognizing sequence 5’-GGWCC-3’ in a haemolysin-less mutant of vibrio parahaemolyticus

Harumi Ueno, Akihiko Kita, Michiko Miyahara, Naoko Ishiwata, Katsutoshi Mise, Ikunoshin Kato, Yoshizumi Ishino

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Abstract

High restriction endonuclease activity was found in a haemolysin-less mutant of the Vibrio parahaemolyticus 1743-1 strain. The endonuclease, named VpaK11BI, recognized the palindromic pentanucleotide sequence of 5′-GGWCC-3′ and cleaved double-stranded DNA after the first G, which is exactly the same as the specificity of AvaII. The haemolysin-less mutant of V. parahaemolyticus is now available for producing the valuable restriction endonuclease on a commercial scale.

Original languageEnglish
Pages (from-to)2129-2130
Number of pages2
JournalBioscience, Biotechnology and Biochemistry
Volume61
Issue number12
DOIs
Publication statusPublished - Jan 1997
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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