Production of new restriction endonuclease Vpa K111BI recognizing sequence 5’-GGWCC-3’ in a haemolysin-less mutant of vibrio parahaemolyticus

Harumi Ueno, Akihiko Kita, Michiko Miyahara, Naoko Ishiwata, Katsutoshi Mise, Ikunoshin Kato, Yoshizumi Ishino

Research output: Contribution to journalArticle

Abstract

High restriction endonuclease activity was found in a haemolysin-less mutant of the Vibrio parahaemolyticus 1743-1 strain. The endonuclease, named VpaK11BI, recognized the palindromic pentanucleotide sequence of 5′-GGWCC-3′ and cleaved double-stranded DNA after the first G, which is exactly the same as the specificity of AvaII. The haemolysin-less mutant of V. parahaemolyticus is now available for producing the valuable restriction endonuclease on a commercial scale.

Original languageEnglish
Pages (from-to)2129-2130
Number of pages2
JournalBioscience, Biotechnology and Biochemistry
Volume61
Issue number12
DOIs
Publication statusPublished - Jan 1 1997

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Vibrio parahaemolyticus
Hemolysin Proteins
DNA Restriction Enzymes
Endonucleases
DNA

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Production of new restriction endonuclease Vpa K111BI recognizing sequence 5’-GGWCC-3’ in a haemolysin-less mutant of vibrio parahaemolyticus. / Ueno, Harumi; Kita, Akihiko; Miyahara, Michiko; Ishiwata, Naoko; Mise, Katsutoshi; Kato, Ikunoshin; Ishino, Yoshizumi.

In: Bioscience, Biotechnology and Biochemistry, Vol. 61, No. 12, 01.01.1997, p. 2129-2130.

Research output: Contribution to journalArticle

Ueno, Harumi ; Kita, Akihiko ; Miyahara, Michiko ; Ishiwata, Naoko ; Mise, Katsutoshi ; Kato, Ikunoshin ; Ishino, Yoshizumi. / Production of new restriction endonuclease Vpa K111BI recognizing sequence 5’-GGWCC-3’ in a haemolysin-less mutant of vibrio parahaemolyticus. In: Bioscience, Biotechnology and Biochemistry. 1997 ; Vol. 61, No. 12. pp. 2129-2130.
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AU - Ishino, Yoshizumi

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