Production of recombinant tumor necrosis factor receptor/Fc fusion protein by genetically manipulated chickens

Kenji Kyogoku, Kazuhiro Yoshida, Hiroyuki Watanabe, Takashi Yamashita, Yoshinori Kawabe, Makoto Motono, Ken Ichi Nishijima, Masamichi Kamihira, Shinji Iijima

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

We previously reported the production of recombinant proteins using genetically manipulated chickens and quails. In this study, we constructed a retroviral vector encoding an expression cassette for a fusion protein of the extracellular domain of the human tumor necrosis factor (TNF) receptor 2 and Fc region of human IgG1 (TNFR/Fc), which is expected as an effective drug for inflammatory diseases such as rheumatoid arthritis. The concentrated viral vector was injected into developing chicken embryos. The chickens that hatched stably produced TNFR/Fc in the serum and egg yolk for six months. It appears that the fused protein is transported and accumulated into yolk from the serum, which is mediated by the Fc receptor. The protein purified from the yolk and serum inhibited the cytotoxic activity of TNF-α toward L929 cells, indicating that the protein produced by the chickens is biologically active. These results indicate the effectiveness of the recovery of Fc-fused proteins from the yolk of genetically manipulated chickens.

Original languageEnglish
Pages (from-to)454-459
Number of pages6
JournalJournal of Bioscience and Bioengineering
Volume105
Issue number5
DOIs
Publication statusPublished - May 2008
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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