Production of scFv-Fc fusion protein using genetically manipulated quails

Yoshinori Kawabe, Masamichi Kamihira, Ken ichiro Ono, Kenji Kyogoku, Ken ichi Nishijima, Shinji Iijima

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

The use of transgenic avian species as a transgenic bioreactor for the production of recombinant proteins has been proposed. In recent years, although various procedures for generating transgenic chickens have been reported, the expression of a useful protein at a commercially feasible level has rarely been attained. In this study, we injected a concentrated retroviral vector into quail embryos to generate genetically manipulated quails that produce recombinant proteins. We found that transgene expression in the whole body at a high level was observed for viral injection into the heart of the developing embryos after a 48-h incubation. For the practical production of a useful protein, a retroviral vector encoding an anti-prion scFv-Fc gene under the control of the β-actin promoter was injected into quail embryos. The quails that hatched stably produced scFv-Fc at a high level in their serum and egg white. The production of scFv-Fc was maintained throughout the breeding period. scFv-Fc purified from the egg white retained the antigen-binding activity. This system exhibited the potential of transgenic quails for the commercial production of recombinant proteins.

Original languageEnglish
Pages (from-to)297-303
Number of pages7
JournalJournal of Bioscience and Bioengineering
Volume102
Issue number4
DOIs
Publication statusPublished - Oct 2006
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Fingerprint Dive into the research topics of 'Production of scFv-Fc fusion protein using genetically manipulated quails'. Together they form a unique fingerprint.

Cite this