Production of tetraploid forms of acid citrus cultivars by top grafting of shoots with sprouting axially buds treated with colchicine

Akira Wakana, Nobuaki Hanada, Sung Min Park, Isao Fukudome, Kohei Kajiwara

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

To induce the tetraploid forms of three diploid acid Citrus cultivars (2n=2x=18), 'Yuzu' (C. junos), 'Kizu'(C. kizu) and 'Hanayu' (C. hanaju), sections of one-year-old spring shoots with sprouting axillary buds were treated with various concentrations of colchicine and immediately top-grafted on 30-year-old satsuma mandarin trees at the beginning of April. Characteristics of the plants developed from the top grafted scions were scored five years after the treatments. Tetraploid forms of the cultivars, which were certified by tetraploid chromosome number in their seedlings, were derived from the shoot sections treated with 0.05-0.4% colchicine solutions for 12 hours for 'Yuzu', 0.05-0.4% for 6 hours for 'Kizu' and 0.1 and 0.2% for 6 hours for 'Hanayu'. As compared with the original plants, these colchicine-induced tetraploid plants produced large flowers, fruits with thick rind, large oil gland and large seeds, and showed high leaf shape index (leaf width / leaf length). The large seeds from open-pollinated tetraploid plants showed the same degree of polyembryony as seeds from their original diploid cultivars, and their seedlings were tetraploid. These results suggested not only that the tetraploid forms of the three cultivars can be directly used for breeding of triploid seedless acid citrus cultivars as seed or pollen parents, but also that the present method is superior to the other methods to produce citrus tetraploid forms directly applicable for triploid breeding at two to four years after colchicine treatments.

Original languageEnglish
Pages (from-to)93-102
Number of pages10
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume50
Issue number1
Publication statusPublished - Feb 2005

Fingerprint

Tetraploidy
Citrus
colchicine
Colchicine
grafting (plants)
sprouting
tetraploidy
buds
Citrus junos
Acids
shoots
acids
cultivars
Seeds
Triploidy
triploidy
seeds
Diploidy
Seedlings
Breeding

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

Cite this

Production of tetraploid forms of acid citrus cultivars by top grafting of shoots with sprouting axially buds treated with colchicine. / Wakana, Akira; Hanada, Nobuaki; Park, Sung Min; Fukudome, Isao; Kajiwara, Kohei.

In: Journal of the Faculty of Agriculture, Kyushu University, Vol. 50, No. 1, 02.2005, p. 93-102.

Research output: Contribution to journalArticle

Wakana, Akira ; Hanada, Nobuaki ; Park, Sung Min ; Fukudome, Isao ; Kajiwara, Kohei. / Production of tetraploid forms of acid citrus cultivars by top grafting of shoots with sprouting axially buds treated with colchicine. In: Journal of the Faculty of Agriculture, Kyushu University. 2005 ; Vol. 50, No. 1. pp. 93-102.
@article{b2a6198d572b416497cd2abde705ae4e,
title = "Production of tetraploid forms of acid citrus cultivars by top grafting of shoots with sprouting axially buds treated with colchicine",
abstract = "To induce the tetraploid forms of three diploid acid Citrus cultivars (2n=2x=18), 'Yuzu' (C. junos), 'Kizu'(C. kizu) and 'Hanayu' (C. hanaju), sections of one-year-old spring shoots with sprouting axillary buds were treated with various concentrations of colchicine and immediately top-grafted on 30-year-old satsuma mandarin trees at the beginning of April. Characteristics of the plants developed from the top grafted scions were scored five years after the treatments. Tetraploid forms of the cultivars, which were certified by tetraploid chromosome number in their seedlings, were derived from the shoot sections treated with 0.05-0.4{\%} colchicine solutions for 12 hours for 'Yuzu', 0.05-0.4{\%} for 6 hours for 'Kizu' and 0.1 and 0.2{\%} for 6 hours for 'Hanayu'. As compared with the original plants, these colchicine-induced tetraploid plants produced large flowers, fruits with thick rind, large oil gland and large seeds, and showed high leaf shape index (leaf width / leaf length). The large seeds from open-pollinated tetraploid plants showed the same degree of polyembryony as seeds from their original diploid cultivars, and their seedlings were tetraploid. These results suggested not only that the tetraploid forms of the three cultivars can be directly used for breeding of triploid seedless acid citrus cultivars as seed or pollen parents, but also that the present method is superior to the other methods to produce citrus tetraploid forms directly applicable for triploid breeding at two to four years after colchicine treatments.",
author = "Akira Wakana and Nobuaki Hanada and Park, {Sung Min} and Isao Fukudome and Kohei Kajiwara",
year = "2005",
month = "2",
language = "English",
volume = "50",
pages = "93--102",
journal = "Journal of the Faculty of Agriculture, Kyushu University",
issn = "0023-6152",
publisher = "Faculty of Agriculture, Kyushu University",
number = "1",

}

TY - JOUR

T1 - Production of tetraploid forms of acid citrus cultivars by top grafting of shoots with sprouting axially buds treated with colchicine

AU - Wakana, Akira

AU - Hanada, Nobuaki

AU - Park, Sung Min

AU - Fukudome, Isao

AU - Kajiwara, Kohei

PY - 2005/2

Y1 - 2005/2

N2 - To induce the tetraploid forms of three diploid acid Citrus cultivars (2n=2x=18), 'Yuzu' (C. junos), 'Kizu'(C. kizu) and 'Hanayu' (C. hanaju), sections of one-year-old spring shoots with sprouting axillary buds were treated with various concentrations of colchicine and immediately top-grafted on 30-year-old satsuma mandarin trees at the beginning of April. Characteristics of the plants developed from the top grafted scions were scored five years after the treatments. Tetraploid forms of the cultivars, which were certified by tetraploid chromosome number in their seedlings, were derived from the shoot sections treated with 0.05-0.4% colchicine solutions for 12 hours for 'Yuzu', 0.05-0.4% for 6 hours for 'Kizu' and 0.1 and 0.2% for 6 hours for 'Hanayu'. As compared with the original plants, these colchicine-induced tetraploid plants produced large flowers, fruits with thick rind, large oil gland and large seeds, and showed high leaf shape index (leaf width / leaf length). The large seeds from open-pollinated tetraploid plants showed the same degree of polyembryony as seeds from their original diploid cultivars, and their seedlings were tetraploid. These results suggested not only that the tetraploid forms of the three cultivars can be directly used for breeding of triploid seedless acid citrus cultivars as seed or pollen parents, but also that the present method is superior to the other methods to produce citrus tetraploid forms directly applicable for triploid breeding at two to four years after colchicine treatments.

AB - To induce the tetraploid forms of three diploid acid Citrus cultivars (2n=2x=18), 'Yuzu' (C. junos), 'Kizu'(C. kizu) and 'Hanayu' (C. hanaju), sections of one-year-old spring shoots with sprouting axillary buds were treated with various concentrations of colchicine and immediately top-grafted on 30-year-old satsuma mandarin trees at the beginning of April. Characteristics of the plants developed from the top grafted scions were scored five years after the treatments. Tetraploid forms of the cultivars, which were certified by tetraploid chromosome number in their seedlings, were derived from the shoot sections treated with 0.05-0.4% colchicine solutions for 12 hours for 'Yuzu', 0.05-0.4% for 6 hours for 'Kizu' and 0.1 and 0.2% for 6 hours for 'Hanayu'. As compared with the original plants, these colchicine-induced tetraploid plants produced large flowers, fruits with thick rind, large oil gland and large seeds, and showed high leaf shape index (leaf width / leaf length). The large seeds from open-pollinated tetraploid plants showed the same degree of polyembryony as seeds from their original diploid cultivars, and their seedlings were tetraploid. These results suggested not only that the tetraploid forms of the three cultivars can be directly used for breeding of triploid seedless acid citrus cultivars as seed or pollen parents, but also that the present method is superior to the other methods to produce citrus tetraploid forms directly applicable for triploid breeding at two to four years after colchicine treatments.

UR - http://www.scopus.com/inward/record.url?scp=15044348322&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=15044348322&partnerID=8YFLogxK

M3 - Article

VL - 50

SP - 93

EP - 102

JO - Journal of the Faculty of Agriculture, Kyushu University

JF - Journal of the Faculty of Agriculture, Kyushu University

SN - 0023-6152

IS - 1

ER -