Production of the oocyte maturation-inducing substance of starfish by heat treatment of S-adenosylmethionine

Masatoshi Mita, Ikuo Yasumasu, Mineo Saneyoshi, Michiyasu Yoshikuni, Yoshitaka Nagahama

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

1-Methyladenine (1-MeA) has been identified as the oocyte maturation-inducing substance (MIS) in starfish, but little is known about its biosynthesis. This study showed that starfish MIS activity was present in a reactant derived from S-adenosylmethionine (SAM) by heat treatment. In vitro MIS production was markedly dependent on the temperature of the SAM solution: it increased as the temperature was raised, and reached a plateau within 5 min upon boiling, although hardly only MIS was observed upon incubation below 20°C. MIS production was also dependent on the solution pH. Analyses by high-performance liquid chromatography and thin-layer chromatography showed that the MIS was 1-MeA, though the maximum amount of 1-MeA obtained from SAM by boiling was only 0.3% of the initial SAM amount. Furthermore, use of S-[14C-methyl]SAM showed that a methyl group of 1-MeA was transferred from the SAM. Thus, it is possible that 1-MeA may be produced from SAM in vivo.

Original languageEnglish
Pages (from-to)117-122
Number of pages6
JournalZoological science
Volume15
Issue number1
DOIs
Publication statusPublished - Feb 1998
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology

Fingerprint Dive into the research topics of 'Production of the oocyte maturation-inducing substance of starfish by heat treatment of S-adenosylmethionine'. Together they form a unique fingerprint.

  • Cite this