Production of transgenic quails with high frequency of germ-line transmission using VSV-G pseudotyped retroviral vector

Shinji Mizuarai, Ken ichiro Ono, Kazuhisa Yamaguchi, Ken ichi Nishijima, Masamichi Kamihira, Shinji Iijima

Research output: Contribution to journalArticlepeer-review

70 Citations (Scopus)

Abstract

We report here the production of transgenic quails using a replication-defective pantropic retroviral vector based on Moloney murine leukemia virus (MoMLV) pseudotyped with vesicular stomatitis virus G protein (VSV-G). The retroviral vector was injected into laid quail embryos at the blastodermal stage, and the embryos were incubated to hatch to produce G0 transgenic quails. Among 134 embryos subjected to viral injection, 37 hatched. The viral vector sequence was detected in the tissues of all Go quails. The germ-line transmission efficiency of Go quails mated with non-transgenic quails was more than 80% on average. Southern blot analysis revealed that the G1 transgenic progeny had one to three copies of the transgene. The expression of vector-encoded neomycin-resistance gene under the control of the Rous sarcoma virus (RSV) promoter was observed in several tissues including heart and muscle of both G1 and G2 transgenic offspring. Due to the high frequency of germ-line transmission, this method may markedly facilitate the production of transgenic avian.

Original languageEnglish
Pages (from-to)456-463
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume286
Issue number3
DOIs
Publication statusPublished - 2001
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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