Production of transgenic rice plants expressing Dioscorea batatas tuber lectin 1 to confer resistance against brown planthopper

Shoichiro Yoshimura, Masaaki Komatsu, Koichiro Kaku, Masatoshi Hori, Tomohisa Ogawa, Koji Muramoto, Tomohiko Kazama, Yukihiro Ito, Kinya Toriyama

Research output: Contribution to journalComment/debate

Abstract

Dioscorea batatas tuber lectin 1 (DB1) is a storage protein isolated from yam tuber and has been shown to be a mannose-binding lectin. Here we produced transgenic rice plants expressing cDNA of DB1 under the control of phloemspecific promoter of rice sucrose synthase-1 gene. DB1 accumulated at a level of 0.07% of total soluble protein. We then evaluated its efficacy for brown planthopper (BPH). After releasing the first instar of BPH on the transgenic rice plants, the number of survived BPH adults was reduced up to 30% compared to that of wild-type rice. The number of the next generation BPH was suppressed to 22% on average in the seven most-resistant plants compared to that of wild-type rice plants when female adult BPH was inoculated. These results demonstrated that DB1 is effective to confer BPH resistance in terms of decreased survival and fecundity.

Original languageEnglish
Pages (from-to)501-504
Number of pages4
JournalPlant Biotechnology
Volume29
Issue number5
DOIs
Publication statusPublished - Dec 1 2012
Externally publishedYes

Fingerprint

Dioscorea polystachya
Dioscorea
Nilaparvata lugens
Genetically Modified Plants
Lectins
lectins
tubers
genetically modified organisms
rice
Mannose-Binding Lectin
sucrose synthase
yams
Fertility
storage proteins
mannose
Proteins
Complementary DNA
Oryza
fecundity
instars

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science
  • Plant Science

Cite this

Production of transgenic rice plants expressing Dioscorea batatas tuber lectin 1 to confer resistance against brown planthopper. / Yoshimura, Shoichiro; Komatsu, Masaaki; Kaku, Koichiro; Hori, Masatoshi; Ogawa, Tomohisa; Muramoto, Koji; Kazama, Tomohiko; Ito, Yukihiro; Toriyama, Kinya.

In: Plant Biotechnology, Vol. 29, No. 5, 01.12.2012, p. 501-504.

Research output: Contribution to journalComment/debate

Yoshimura, Shoichiro ; Komatsu, Masaaki ; Kaku, Koichiro ; Hori, Masatoshi ; Ogawa, Tomohisa ; Muramoto, Koji ; Kazama, Tomohiko ; Ito, Yukihiro ; Toriyama, Kinya. / Production of transgenic rice plants expressing Dioscorea batatas tuber lectin 1 to confer resistance against brown planthopper. In: Plant Biotechnology. 2012 ; Vol. 29, No. 5. pp. 501-504.
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AU - Komatsu, Masaaki

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AU - Hori, Masatoshi

AU - Ogawa, Tomohisa

AU - Muramoto, Koji

AU - Kazama, Tomohiko

AU - Ito, Yukihiro

AU - Toriyama, Kinya

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