Profiling of bacterial flora in crevices around titanium orthodontic anchor plates

Renya Sato, Takuichi Sato, Ichiro Takahashi, Junji Sugawara, Nobuhiro Takahashi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Objectives: The aims of this study were to characterize the microflora in crevices around titanium orthodontic anchor plates using anaerobic culture and molecular biological techniques for bacterial identification, and to compare the microbial composition between crevices around anchor plates and gingival crevices. Material and methods: Samples from crevices around titanium anchor plates and healthy gingival crevices of 17 subjects (aged 20-29) were cultured anaerobically, and isolated bacteria were identified by 16S rRNA sequencing. Results: The average logarithm colony-forming units/ml were 6.84, 7.51 and 8.88 in healthy anchor plate crevices, inflamed anchor plate crevices and healthy gingival crevices, respectively, indicating that the bacterial density of anchor plate crevices was lower than that of healthy gingival crevices. Of 184 strains isolated from healthy anchor plate crevices of seven subjects, 108 (59%) were anaerobic bacteria, while 73 (40%) were facultative bacteria. Predominant isolates were Gram-negative rods, such as Campylobacter (12%), Fusobacterium (10%) and Selenomonas (10%), and Gram-positive facultative bacteria, such as Actinomyces (17%) and Streptococcus (8.2%). Of 133 strains isolated from inflamed anchor plate crevices of three subjects, 110 (83%) were anaerobic bacteria, while predominant isolates were Gram-negative rods, such as Prevotella (47%), Fusobacterium (33%) and Campylobacter (16%). On the other hand, of 146 strains isolated from healthy gingival crevices of seven subjects, 98 (67%) were facultative bacteria, while 45 (31%) were anaerobic bacteria. Predominant isolates were Gram-positive facultative bacteria, such as Actinomyces (37%) and Streptococcus (20%). Conclusions: These results suggest that the environment in crevices around titanium orthodontic anchor plates is anaerobic and supportive of anaerobic growth of bacteria, which may trigger inflammation in the tissue around the plates.

Original languageEnglish
Pages (from-to)21-26
Number of pages6
JournalClinical Oral Implants Research
Volume18
Issue number1
DOIs
Publication statusPublished - Feb 1 2007
Externally publishedYes

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Anaerobic Bacteria
Orthodontics
Titanium
Fusobacterium
Actinomyces
Campylobacter
Gram-Positive Bacteria
Streptococcus
Bacteria
Selenomonas
Prevotella
Stem Cells
Inflammation
Growth

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

Profiling of bacterial flora in crevices around titanium orthodontic anchor plates. / Sato, Renya; Sato, Takuichi; Takahashi, Ichiro; Sugawara, Junji; Takahashi, Nobuhiro.

In: Clinical Oral Implants Research, Vol. 18, No. 1, 01.02.2007, p. 21-26.

Research output: Contribution to journalArticle

Sato, Renya ; Sato, Takuichi ; Takahashi, Ichiro ; Sugawara, Junji ; Takahashi, Nobuhiro. / Profiling of bacterial flora in crevices around titanium orthodontic anchor plates. In: Clinical Oral Implants Research. 2007 ; Vol. 18, No. 1. pp. 21-26.
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abstract = "Objectives: The aims of this study were to characterize the microflora in crevices around titanium orthodontic anchor plates using anaerobic culture and molecular biological techniques for bacterial identification, and to compare the microbial composition between crevices around anchor plates and gingival crevices. Material and methods: Samples from crevices around titanium anchor plates and healthy gingival crevices of 17 subjects (aged 20-29) were cultured anaerobically, and isolated bacteria were identified by 16S rRNA sequencing. Results: The average logarithm colony-forming units/ml were 6.84, 7.51 and 8.88 in healthy anchor plate crevices, inflamed anchor plate crevices and healthy gingival crevices, respectively, indicating that the bacterial density of anchor plate crevices was lower than that of healthy gingival crevices. Of 184 strains isolated from healthy anchor plate crevices of seven subjects, 108 (59{\%}) were anaerobic bacteria, while 73 (40{\%}) were facultative bacteria. Predominant isolates were Gram-negative rods, such as Campylobacter (12{\%}), Fusobacterium (10{\%}) and Selenomonas (10{\%}), and Gram-positive facultative bacteria, such as Actinomyces (17{\%}) and Streptococcus (8.2{\%}). Of 133 strains isolated from inflamed anchor plate crevices of three subjects, 110 (83{\%}) were anaerobic bacteria, while predominant isolates were Gram-negative rods, such as Prevotella (47{\%}), Fusobacterium (33{\%}) and Campylobacter (16{\%}). On the other hand, of 146 strains isolated from healthy gingival crevices of seven subjects, 98 (67{\%}) were facultative bacteria, while 45 (31{\%}) were anaerobic bacteria. Predominant isolates were Gram-positive facultative bacteria, such as Actinomyces (37{\%}) and Streptococcus (20{\%}). Conclusions: These results suggest that the environment in crevices around titanium orthodontic anchor plates is anaerobic and supportive of anaerobic growth of bacteria, which may trigger inflammation in the tissue around the plates.",
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