Prognostic implication of p53 protein expression in relation to nuclear pleomorphism and the MIB-1 counts in breast cancer

Shinichi Tsutsui, Kazuhiro Yasuda, Hidefumi Higashi, Kouichirou Tahara, Satoshi Sugita, Hidetoshi Eguchi, Hiroto Kayashima, Nobuhiko Miyazaki, Yoichi Muto, Shoichi Era

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Abstract

Background: A close correlation of the p53 protein expression to nuclear pleomorphism and proliferative activity in breast cancer has been reported. The prognostic implications of p53 protein expression, however, in relation to nuclear pleomorphism and proliferative activity in breast cancer remain controversial. Patients and Methods: Nuclear pleomorphism and immunohistochemical reactivity for p53 protein and MIB-1 were evaluated on formalin-fixed paraffin-stored sections from 250 patients with breast cancer for whom the median follow-up duration was 6.4 years. Results: P53 protein expression was positive in 66 (26.4%) of 250 cases. Nuclear pleomorphism was grade I or 11 in 169 (67.6%) cases and grade 111 in 81 (32.4%) cases. The MIB-1 counts were more than 10% in 102 (40.8%) cases and less than 10% in 148 (59.2%) cases. There was a close correlation between p53 protein expression and nuclear pleomorphism (p < 0.0001) and between p53 protein expression and MIB-1 counts (p < 0.0001). Univariate analyses showed the 66 cases with positive p53 protein expression to have a significantly (p = 0.0284) worse disease free survival (DFS) than the 184 cases with negative p53 protein expression. A multivariate analysis, however, on the variables including all of p53 protein expression, nuclear pleomorphism and MIB-1 counts indicated the MIB-1 counts (p = 0.0041) as well as the lymph node status to be independently significant factors for DFS, while neither p53 protein expression nor nuclear pleomorphism were independently significant factors for DFS. Conclusion: The present study demonstrated that the p53 protein expression, nuclear pleomorphism and MIB-1 counts all demonstrated prognostic significance for breast cancer, while the most significant prognostic indicator among these three biological parameters was the MIB-1 counts.

Original languageEnglish
Pages (from-to)160-168
Number of pages9
JournalBreast Cancer
Volume11
Issue number2
DOIs
Publication statusPublished - Apr 1 2004
Externally publishedYes

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Breast Neoplasms
Nuclear Proteins
Ki-67 Antigen
Disease-Free Survival
Proteins
Paraffin
Formaldehyde
Multivariate Analysis
Lymph Nodes

All Science Journal Classification (ASJC) codes

  • Oncology
  • Radiology Nuclear Medicine and imaging
  • Pharmacology (medical)

Cite this

Tsutsui, S., Yasuda, K., Higashi, H., Tahara, K., Sugita, S., Eguchi, H., ... Era, S. (2004). Prognostic implication of p53 protein expression in relation to nuclear pleomorphism and the MIB-1 counts in breast cancer. Breast Cancer, 11(2), 160-168. https://doi.org/10.1007/BF02968296

Prognostic implication of p53 protein expression in relation to nuclear pleomorphism and the MIB-1 counts in breast cancer. / Tsutsui, Shinichi; Yasuda, Kazuhiro; Higashi, Hidefumi; Tahara, Kouichirou; Sugita, Satoshi; Eguchi, Hidetoshi; Kayashima, Hiroto; Miyazaki, Nobuhiko; Muto, Yoichi; Era, Shoichi.

In: Breast Cancer, Vol. 11, No. 2, 01.04.2004, p. 160-168.

Research output: Contribution to journalArticle

Tsutsui, S, Yasuda, K, Higashi, H, Tahara, K, Sugita, S, Eguchi, H, Kayashima, H, Miyazaki, N, Muto, Y & Era, S 2004, 'Prognostic implication of p53 protein expression in relation to nuclear pleomorphism and the MIB-1 counts in breast cancer', Breast Cancer, vol. 11, no. 2, pp. 160-168. https://doi.org/10.1007/BF02968296
Tsutsui, Shinichi ; Yasuda, Kazuhiro ; Higashi, Hidefumi ; Tahara, Kouichirou ; Sugita, Satoshi ; Eguchi, Hidetoshi ; Kayashima, Hiroto ; Miyazaki, Nobuhiko ; Muto, Yoichi ; Era, Shoichi. / Prognostic implication of p53 protein expression in relation to nuclear pleomorphism and the MIB-1 counts in breast cancer. In: Breast Cancer. 2004 ; Vol. 11, No. 2. pp. 160-168.
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abstract = "Background: A close correlation of the p53 protein expression to nuclear pleomorphism and proliferative activity in breast cancer has been reported. The prognostic implications of p53 protein expression, however, in relation to nuclear pleomorphism and proliferative activity in breast cancer remain controversial. Patients and Methods: Nuclear pleomorphism and immunohistochemical reactivity for p53 protein and MIB-1 were evaluated on formalin-fixed paraffin-stored sections from 250 patients with breast cancer for whom the median follow-up duration was 6.4 years. Results: P53 protein expression was positive in 66 (26.4{\%}) of 250 cases. Nuclear pleomorphism was grade I or 11 in 169 (67.6{\%}) cases and grade 111 in 81 (32.4{\%}) cases. The MIB-1 counts were more than 10{\%} in 102 (40.8{\%}) cases and less than 10{\%} in 148 (59.2{\%}) cases. There was a close correlation between p53 protein expression and nuclear pleomorphism (p < 0.0001) and between p53 protein expression and MIB-1 counts (p < 0.0001). Univariate analyses showed the 66 cases with positive p53 protein expression to have a significantly (p = 0.0284) worse disease free survival (DFS) than the 184 cases with negative p53 protein expression. A multivariate analysis, however, on the variables including all of p53 protein expression, nuclear pleomorphism and MIB-1 counts indicated the MIB-1 counts (p = 0.0041) as well as the lymph node status to be independently significant factors for DFS, while neither p53 protein expression nor nuclear pleomorphism were independently significant factors for DFS. Conclusion: The present study demonstrated that the p53 protein expression, nuclear pleomorphism and MIB-1 counts all demonstrated prognostic significance for breast cancer, while the most significant prognostic indicator among these three biological parameters was the MIB-1 counts.",
author = "Shinichi Tsutsui and Kazuhiro Yasuda and Hidefumi Higashi and Kouichirou Tahara and Satoshi Sugita and Hidetoshi Eguchi and Hiroto Kayashima and Nobuhiko Miyazaki and Yoichi Muto and Shoichi Era",
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T1 - Prognostic implication of p53 protein expression in relation to nuclear pleomorphism and the MIB-1 counts in breast cancer

AU - Tsutsui, Shinichi

AU - Yasuda, Kazuhiro

AU - Higashi, Hidefumi

AU - Tahara, Kouichirou

AU - Sugita, Satoshi

AU - Eguchi, Hidetoshi

AU - Kayashima, Hiroto

AU - Miyazaki, Nobuhiko

AU - Muto, Yoichi

AU - Era, Shoichi

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N2 - Background: A close correlation of the p53 protein expression to nuclear pleomorphism and proliferative activity in breast cancer has been reported. The prognostic implications of p53 protein expression, however, in relation to nuclear pleomorphism and proliferative activity in breast cancer remain controversial. Patients and Methods: Nuclear pleomorphism and immunohistochemical reactivity for p53 protein and MIB-1 were evaluated on formalin-fixed paraffin-stored sections from 250 patients with breast cancer for whom the median follow-up duration was 6.4 years. Results: P53 protein expression was positive in 66 (26.4%) of 250 cases. Nuclear pleomorphism was grade I or 11 in 169 (67.6%) cases and grade 111 in 81 (32.4%) cases. The MIB-1 counts were more than 10% in 102 (40.8%) cases and less than 10% in 148 (59.2%) cases. There was a close correlation between p53 protein expression and nuclear pleomorphism (p < 0.0001) and between p53 protein expression and MIB-1 counts (p < 0.0001). Univariate analyses showed the 66 cases with positive p53 protein expression to have a significantly (p = 0.0284) worse disease free survival (DFS) than the 184 cases with negative p53 protein expression. A multivariate analysis, however, on the variables including all of p53 protein expression, nuclear pleomorphism and MIB-1 counts indicated the MIB-1 counts (p = 0.0041) as well as the lymph node status to be independently significant factors for DFS, while neither p53 protein expression nor nuclear pleomorphism were independently significant factors for DFS. Conclusion: The present study demonstrated that the p53 protein expression, nuclear pleomorphism and MIB-1 counts all demonstrated prognostic significance for breast cancer, while the most significant prognostic indicator among these three biological parameters was the MIB-1 counts.

AB - Background: A close correlation of the p53 protein expression to nuclear pleomorphism and proliferative activity in breast cancer has been reported. The prognostic implications of p53 protein expression, however, in relation to nuclear pleomorphism and proliferative activity in breast cancer remain controversial. Patients and Methods: Nuclear pleomorphism and immunohistochemical reactivity for p53 protein and MIB-1 were evaluated on formalin-fixed paraffin-stored sections from 250 patients with breast cancer for whom the median follow-up duration was 6.4 years. Results: P53 protein expression was positive in 66 (26.4%) of 250 cases. Nuclear pleomorphism was grade I or 11 in 169 (67.6%) cases and grade 111 in 81 (32.4%) cases. The MIB-1 counts were more than 10% in 102 (40.8%) cases and less than 10% in 148 (59.2%) cases. There was a close correlation between p53 protein expression and nuclear pleomorphism (p < 0.0001) and between p53 protein expression and MIB-1 counts (p < 0.0001). Univariate analyses showed the 66 cases with positive p53 protein expression to have a significantly (p = 0.0284) worse disease free survival (DFS) than the 184 cases with negative p53 protein expression. A multivariate analysis, however, on the variables including all of p53 protein expression, nuclear pleomorphism and MIB-1 counts indicated the MIB-1 counts (p = 0.0041) as well as the lymph node status to be independently significant factors for DFS, while neither p53 protein expression nor nuclear pleomorphism were independently significant factors for DFS. Conclusion: The present study demonstrated that the p53 protein expression, nuclear pleomorphism and MIB-1 counts all demonstrated prognostic significance for breast cancer, while the most significant prognostic indicator among these three biological parameters was the MIB-1 counts.

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