Proliferation and differentiation of cultured MC3T3-E1 osteoblasts on surface-layer modified hydroxyapatite ceramic with acid and heat treatments

Tetsuya Yuasa, Youji Miyamoto, Masayuki Kon, Kunio Ishikawa, Masaaki Takechi, Yukihiro Momota, Seiko Tatehara, Hideyuki Takano, Shiho Mimamiguchi, Masaru Nagayama

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8 Citations (Scopus)

Abstract

Effects of functionally gradient calcium phosphate consisting of hydroxyapatite (HAP) and α-tricalcium phosphate (α-TCP) on proliferation and differentiation of osteoblasts were evaluated using MC3T3-E1 cells. There were no significant differences in the proliferation of MC3T3-E1 cells among HAP- α-TCP functionally gradient calcium phosphate, pure HAP, and cell culture plastic wells. mRNA expressions of type I collagen, alkaline phosphate, and osteocalcine were evaluated as indexes of initial, mid-stage, and late-stage osteoblastic differentiation. Basically, HAP- α-TCP functionally gradient calcium phosphate and pure HAP enhanced the expressions of the three markers when compared with that of cell culture plastic wells. For type I collagen and alkaline phosphate expressions, HAP- α-TCP functionally gradient calcium phosphate showed the same expression level as pure HAP. For osteocalcine expression, HAP- α-TCP functionally gradient calcium phosphate showed a higher level than pure HAP. We concluded, therefore, HAP- α-TCP functionally gradient calcium phosphate has good potential to be a bone filler material with high osteoconductivity.

Original languageEnglish
Pages (from-to)207-212
Number of pages6
Journaldental materials journal
Volume24
Issue number2
DOIs
Publication statusPublished - Jun 2005

All Science Journal Classification (ASJC) codes

  • Ceramics and Composites
  • Dentistry(all)

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    Yuasa, T., Miyamoto, Y., Kon, M., Ishikawa, K., Takechi, M., Momota, Y., Tatehara, S., Takano, H., Mimamiguchi, S., & Nagayama, M. (2005). Proliferation and differentiation of cultured MC3T3-E1 osteoblasts on surface-layer modified hydroxyapatite ceramic with acid and heat treatments. dental materials journal, 24(2), 207-212. https://doi.org/10.4012/dmj.24.207