Proliferation and stratification of keratinocyte on cultured amniotic epithelial cells for tissue engineering

Akira Ito, Yohei Takizawa, Masashige Shinkai, Hiroyuki Honda, Ken Ichiro Hata, Minoru Ueda, Naohiko Kuno, Atsuo Itakura, Takeshi Kobayashi

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7 Citations (Scopus)

Abstract

Human amniotic epithelial cells (HAECs) are formed from amnioblasts, separated from the epiblast at about the 8th day after fertilization. Recent studies suggest that HAECs can produce various biologically active substances. In this study, the effects of cultured HAECs on keratinocytes were investigated. First of all, the effect of the medium conditioned by cultured HAECs on the proliferation of keratinocytes was examined. The conditioned medium significantly enhanced the proliferation (P<0.05). Next, the effect of co-culture with HAECs was also examined. The keratinocytes formed a stratified epithelium on day 7 after the start of co-culture. The cultured epithelium formed by the co-culture was five to six layers thick, could be detached by dispase treatment, and had sufficient strength as a sheet. These results suggest that HAECs will be a novel supplemental material for the tissue engineering of skin.

Original languageEnglish
Pages (from-to)589-593
Number of pages5
JournalJournal of Bioscience and Bioengineering
Volume95
Issue number6
DOIs
Publication statusPublished - Jan 1 2003

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All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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