TY - JOUR
T1 - Proliferation and stratification of keratinocyte on cultured amniotic epithelial cells for tissue engineering
AU - Ito, Akira
AU - Takizawa, Yohei
AU - Shinkai, Masashige
AU - Honda, Hiroyuki
AU - Hata, Ken Ichiro
AU - Ueda, Minoru
AU - Kuno, Naohiko
AU - Itakura, Atsuo
AU - Kobayashi, Takeshi
N1 - Funding Information:
This study was supported in part by a Grant-in-Aid for Scientific Research (no. 13650855) from the Ministry of Education, Culture, Sports, Science and Technology.
PY - 2003
Y1 - 2003
N2 - Human amniotic epithelial cells (HAECs) are formed from amnioblasts, separated from the epiblast at about the 8th day after fertilization. Recent studies suggest that HAECs can produce various biologically active substances. In this study, the effects of cultured HAECs on keratinocytes were investigated. First of all, the effect of the medium conditioned by cultured HAECs on the proliferation of keratinocytes was examined. The conditioned medium significantly enhanced the proliferation (P<0.05). Next, the effect of co-culture with HAECs was also examined. The keratinocytes formed a stratified epithelium on day 7 after the start of co-culture. The cultured epithelium formed by the co-culture was five to six layers thick, could be detached by dispase treatment, and had sufficient strength as a sheet. These results suggest that HAECs will be a novel supplemental material for the tissue engineering of skin.
AB - Human amniotic epithelial cells (HAECs) are formed from amnioblasts, separated from the epiblast at about the 8th day after fertilization. Recent studies suggest that HAECs can produce various biologically active substances. In this study, the effects of cultured HAECs on keratinocytes were investigated. First of all, the effect of the medium conditioned by cultured HAECs on the proliferation of keratinocytes was examined. The conditioned medium significantly enhanced the proliferation (P<0.05). Next, the effect of co-culture with HAECs was also examined. The keratinocytes formed a stratified epithelium on day 7 after the start of co-culture. The cultured epithelium formed by the co-culture was five to six layers thick, could be detached by dispase treatment, and had sufficient strength as a sheet. These results suggest that HAECs will be a novel supplemental material for the tissue engineering of skin.
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U2 - 10.1016/S1389-1723(03)80167-6
DO - 10.1016/S1389-1723(03)80167-6
M3 - Article
C2 - 16233462
AN - SCOPUS:0038006414
SN - 1389-1723
VL - 95
SP - 589
EP - 593
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
IS - 6
ER -