Abstract
In this study, we have extended a cyclophosphamide (CP)-induced tolerance system to kidney transplantation in rats to examine whether or not we can overcome fully allogeneic (major histocompatibility complex plus minor histocompatibility) antigen barriers in organ transplantation. In the recipient Lewis (LEW, RT11) rats that were primed intravenously with 4 X 108 spleen cells plus 2 X 108 bone marrow cells from Brown-Norway (BN, RT1n) rats and treated intraperitoneally with 100 mg./kg. of cyclophosphamide (CP) 2 days later, the survival of kidney allografts, but not skin allografts, from BN was prolonged as compared with that in the untreated LEW rats. Some of the kidney allografts survived for more than 100 days without further immunosuppressants. The tolerant state induced was tolerogen specific, and the suppression of tissue damage of the grafted kidney in such tolerant rats was also confirmed by the histopathological findings of the grafted kidney. These results indicate that considerable levels of tolerance can be induced, at least in organ transplantation, across fully allogeneic antigen barriers in rats by a CP-induced tolerance system. We believe that the present study is the first step in applying our CP-induced tolerance system using skin grafting in the murine model to clinical organ transplantation.
| Original language | English |
|---|---|
| Pages (from-to) | 1693-1696 |
| Number of pages | 4 |
| Journal | The Journal of urology |
| Volume | 153 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - May 1995 |
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All Science Journal Classification (ASJC) codes
- Urology
Cite this
Prolongation of Kidney Graft Survival by Cyclophosphamide-induced Tolerance in Rats. / Eto, Masatoshi; Nishimura, Yousuke; Matsuo, Kenichi; Omoto, Kazuya; Goto, Ken; Kumuzawa, Joichi; Nomoto, Kikuo.
In: The Journal of urology, Vol. 153, No. 5, 05.1995, p. 1693-1696.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Prolongation of Kidney Graft Survival by Cyclophosphamide-induced Tolerance in Rats
AU - Eto, Masatoshi
AU - Nishimura, Yousuke
AU - Matsuo, Kenichi
AU - Omoto, Kazuya
AU - Goto, Ken
AU - Kumuzawa, Joichi
AU - Nomoto, Kikuo
PY - 1995/5
Y1 - 1995/5
N2 - In this study, we have extended a cyclophosphamide (CP)-induced tolerance system to kidney transplantation in rats to examine whether or not we can overcome fully allogeneic (major histocompatibility complex plus minor histocompatibility) antigen barriers in organ transplantation. In the recipient Lewis (LEW, RT11) rats that were primed intravenously with 4 X 108 spleen cells plus 2 X 108 bone marrow cells from Brown-Norway (BN, RT1n) rats and treated intraperitoneally with 100 mg./kg. of cyclophosphamide (CP) 2 days later, the survival of kidney allografts, but not skin allografts, from BN was prolonged as compared with that in the untreated LEW rats. Some of the kidney allografts survived for more than 100 days without further immunosuppressants. The tolerant state induced was tolerogen specific, and the suppression of tissue damage of the grafted kidney in such tolerant rats was also confirmed by the histopathological findings of the grafted kidney. These results indicate that considerable levels of tolerance can be induced, at least in organ transplantation, across fully allogeneic antigen barriers in rats by a CP-induced tolerance system. We believe that the present study is the first step in applying our CP-induced tolerance system using skin grafting in the murine model to clinical organ transplantation.
AB - In this study, we have extended a cyclophosphamide (CP)-induced tolerance system to kidney transplantation in rats to examine whether or not we can overcome fully allogeneic (major histocompatibility complex plus minor histocompatibility) antigen barriers in organ transplantation. In the recipient Lewis (LEW, RT11) rats that were primed intravenously with 4 X 108 spleen cells plus 2 X 108 bone marrow cells from Brown-Norway (BN, RT1n) rats and treated intraperitoneally with 100 mg./kg. of cyclophosphamide (CP) 2 days later, the survival of kidney allografts, but not skin allografts, from BN was prolonged as compared with that in the untreated LEW rats. Some of the kidney allografts survived for more than 100 days without further immunosuppressants. The tolerant state induced was tolerogen specific, and the suppression of tissue damage of the grafted kidney in such tolerant rats was also confirmed by the histopathological findings of the grafted kidney. These results indicate that considerable levels of tolerance can be induced, at least in organ transplantation, across fully allogeneic antigen barriers in rats by a CP-induced tolerance system. We believe that the present study is the first step in applying our CP-induced tolerance system using skin grafting in the murine model to clinical organ transplantation.
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UR - http://www.scopus.com/inward/citedby.url?scp=0028924473&partnerID=8YFLogxK
U2 - 10.1016/S0022-5347(01)67506-2
DO - 10.1016/S0022-5347(01)67506-2
M3 - Article
C2 - 7715012
AN - SCOPUS:0028924473
VL - 153
SP - 1693
EP - 1696
JO - Journal of Urology
JF - Journal of Urology
SN - 0022-5347
IS - 5
ER -