Prolongation of the active lifetime of a biomolecular motor for in vitro motility assay by using an inert atmosphere

Arif Md Rashedul Kabir, Daisuke Inoue, Akira Kakugo, Akiko Kamei, Jian Ping Gong

Research output: Contribution to journalArticle

Abstract

Over the last few decades, the in vitro motility assay has been performed to probe the biophysical and chemo-mechanical properties as well as the self-organization process of biomolecular motor systems such as actin-myosin and microtubule-kinesin. However, aggression of the reactive oxygen species (ROS) and concomitant termination of the activity of biomolecular motors during investigation remains a drawback of this assay. Despite enzymatic protection that makes use of a combination of glucose, glucose oxidase, and catalase, the active lifetime of biomolecular motors is found to be only a few hours and this short lifetime restricts further study on those systems. We have solved this problem by using a newly developed system of the in vitro motility assay that is conducted in an inert nitrogen gas atmosphere free of ROS. Using microtubule-kinesin as a model system we have shown that our system has prolonged the active lifetime of the biomolecular motor until several days and even a week by protecting it from oxidative damage.

Original languageEnglish
Pages (from-to)13659-13668
Number of pages10
JournalLangmuir
Volume27
Issue number22
DOIs
Publication statusPublished - Nov 15 2011
Externally publishedYes

Fingerprint

prolongation
inert atmosphere
locomotion
Assays
glucose
Kinesin
life (durability)
efferent nervous systems
free atmosphere
myosins
catalase
Reactive Oxygen Species
oxidase
oxygen
Glucose Oxidase
Glucose oxidase
Oxygen
Myosins
mechanical properties
Catalase

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

Cite this

Prolongation of the active lifetime of a biomolecular motor for in vitro motility assay by using an inert atmosphere. / Kabir, Arif Md Rashedul; Inoue, Daisuke; Kakugo, Akira; Kamei, Akiko; Gong, Jian Ping.

In: Langmuir, Vol. 27, No. 22, 15.11.2011, p. 13659-13668.

Research output: Contribution to journalArticle

Kabir, Arif Md Rashedul ; Inoue, Daisuke ; Kakugo, Akira ; Kamei, Akiko ; Gong, Jian Ping. / Prolongation of the active lifetime of a biomolecular motor for in vitro motility assay by using an inert atmosphere. In: Langmuir. 2011 ; Vol. 27, No. 22. pp. 13659-13668.
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