Prolongation of the active lifetime of a biomolecular motor for in vitro motility assay by using an inert atmosphere

Arif Md Rashedul Kabir, Daisuke Inoue, Akira Kakugo, Akiko Kamei, Jian Ping Gong

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Over the last few decades, the in vitro motility assay has been performed to probe the biophysical and chemo-mechanical properties as well as the self-organization process of biomolecular motor systems such as actin-myosin and microtubule-kinesin. However, aggression of the reactive oxygen species (ROS) and concomitant termination of the activity of biomolecular motors during investigation remains a drawback of this assay. Despite enzymatic protection that makes use of a combination of glucose, glucose oxidase, and catalase, the active lifetime of biomolecular motors is found to be only a few hours and this short lifetime restricts further study on those systems. We have solved this problem by using a newly developed system of the in vitro motility assay that is conducted in an inert nitrogen gas atmosphere free of ROS. Using microtubule-kinesin as a model system we have shown that our system has prolonged the active lifetime of the biomolecular motor until several days and even a week by protecting it from oxidative damage.

Original languageEnglish
Pages (from-to)13659-13668
Number of pages10
JournalLangmuir
Volume27
Issue number22
DOIs
Publication statusPublished - Nov 15 2011
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

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