Cell adhesive peptide Arg-Gly-Asp (RGD) was immobilized using ProNectin F (PnF) on a non-treated polystyrene petri dish at a PnF density of 20 ng cm -2, which is sufficient for primary rat hepatocyte immobilization. The density of PnF on the dish affects cell morphology and expression of the differentiated functions within the range of 2-2500 ng cm-2. An optimal monolayer state with defined cell boundaries and hepatocyte nuclei was formed on a 2500 ng cm-2 PnF-coated petri dish, and the ammonia metabolic function was expressed at as high a level as in the hepatocyte/spheroid. We conclude that 2500 ng cm-2 PnF enhances the morphological stability and expression of liver-specific functions of the hepatocyte.
All Science Journal Classification (ASJC) codes
- Applied Microbiology and Biotechnology