Promotion of monolayer formation and high expression of ammonia metabolism of primary rat hepatocytes on arginine-glycine-aspartic acid-containing peptide-coated polystyrene dish

Hiroyuki Ijima, Koei Kawakami

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Cell adhesive peptide Arg-Gly-Asp (RGD) was immobilized using ProNectin F (PnF) on a non-treated polystyrene petri dish at a PnF density of 20 ng cm -2, which is sufficient for primary rat hepatocyte immobilization. The density of PnF on the dish affects cell morphology and expression of the differentiated functions within the range of 2-2500 ng cm-2. An optimal monolayer state with defined cell boundaries and hepatocyte nuclei was formed on a 2500 ng cm-2 PnF-coated petri dish, and the ammonia metabolic function was expressed at as high a level as in the hepatocyte/spheroid. We conclude that 2500 ng cm-2 PnF enhances the morphological stability and expression of liver-specific functions of the hepatocyte.

Original languageEnglish
Pages (from-to)62-66
Number of pages5
JournalJournal of Bioscience and Bioengineering
Volume100
Issue number1
DOIs
Publication statusPublished - Jan 1 2005

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Arginine
Polystyrenes
Ammonia
Metabolism
Aspartic Acid
Glycine
Peptides
Amino acids
Rats
Hepatocytes
Monolayers
Acids
Liver
Adhesives
Immobilization
ProNectin F

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

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AB - Cell adhesive peptide Arg-Gly-Asp (RGD) was immobilized using ProNectin F (PnF) on a non-treated polystyrene petri dish at a PnF density of 20 ng cm -2, which is sufficient for primary rat hepatocyte immobilization. The density of PnF on the dish affects cell morphology and expression of the differentiated functions within the range of 2-2500 ng cm-2. An optimal monolayer state with defined cell boundaries and hepatocyte nuclei was formed on a 2500 ng cm-2 PnF-coated petri dish, and the ammonia metabolic function was expressed at as high a level as in the hepatocyte/spheroid. We conclude that 2500 ng cm-2 PnF enhances the morphological stability and expression of liver-specific functions of the hepatocyte.

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