Properties of a trifluoroleucine-resistant mutant of Saccharomyces cerevisiae

Takahiro Oba, Yoshitsugu Yamamoto, Shuji Nomiyama, Hikaru Suenaga, Shigeru Muta, Kosuke Tashiro, Satoru Kuhara

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We characterized a trifluoroleucine-resistant mutant of Saccharomyces cerevisiae, TFL20, that has a mutation in the LEU4 gene. We monitored the concentration of extracellular i-AmOH and intracellular amino acids, and compared the ratios of gene expression in TFL20 with the wild-type strain, K30. We found that the LEU1, LEU2, and BAT1 genes were up-regulated in TFL20 for metabolism, and that TFL20 simultaneously produced as much i-AmOH and leucine as K30 does.

Original languageEnglish
Pages (from-to)1776-1779
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume70
Issue number7
DOIs
Publication statusPublished - Aug 1 2006

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Yeast
Saccharomyces cerevisiae
Genes
Metabolism
Gene expression
Leucine
Gene Expression
Amino Acids
Mutation

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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Properties of a trifluoroleucine-resistant mutant of Saccharomyces cerevisiae. / Oba, Takahiro; Yamamoto, Yoshitsugu; Nomiyama, Shuji; Suenaga, Hikaru; Muta, Shigeru; Tashiro, Kosuke; Kuhara, Satoru.

In: Bioscience, Biotechnology and Biochemistry, Vol. 70, No. 7, 01.08.2006, p. 1776-1779.

Research output: Contribution to journalArticle

Oba, Takahiro ; Yamamoto, Yoshitsugu ; Nomiyama, Shuji ; Suenaga, Hikaru ; Muta, Shigeru ; Tashiro, Kosuke ; Kuhara, Satoru. / Properties of a trifluoroleucine-resistant mutant of Saccharomyces cerevisiae. In: Bioscience, Biotechnology and Biochemistry. 2006 ; Vol. 70, No. 7. pp. 1776-1779.
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