Property of IK,n in inner hair cells isolated from guinea-pig cochlea

Takashi Kimitsuki, Noritaka Komune, Teppei Noda, Kazutaka Takaiwa, Mitsuru Ohashi, Shizuo Komune

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

One of the potassium currents, IK,n, is already activated at the resting potential of the cell and thus determines the membrane potential. KCNQ4 channel has been identified as the molecular correlate of IK,n. In the present study, we measured IK,n in acutely isolated IHCs of guinea-pig cochlea using the whole-cell voltage-clamp techniques, and investigated the properties of the currents. IK,n was 70% activated around the resting potential of -60 mV and deactivated on hyperpolarization. IK,n was blocked by the KCNQ-channel blockers, linopirdine (100 μM) and XE991 (10 μM), but was insensitive to both IK,f blocker, tetraethylammonium (TEA), and IK,s blocker, 4-aminopyridine (4-AP). There was no significant difference in the size of IK,n between the apical and basal turn IHCs.

Original languageEnglish
Pages (from-to)57-62
Number of pages6
JournalHearing Research
Volume261
Issue number1-2
DOIs
Publication statusPublished - Mar 1 2010

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Inner Auditory Hair Cells
Cochlea
Membrane Potentials
Guinea Pigs
linopirdine
4-Aminopyridine
Tetraethylammonium
Patch-Clamp Techniques
Potassium

All Science Journal Classification (ASJC) codes

  • Sensory Systems

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Property of IK,n in inner hair cells isolated from guinea-pig cochlea. / Kimitsuki, Takashi; Komune, Noritaka; Noda, Teppei; Takaiwa, Kazutaka; Ohashi, Mitsuru; Komune, Shizuo.

In: Hearing Research, Vol. 261, No. 1-2, 01.03.2010, p. 57-62.

Research output: Contribution to journalArticle

Kimitsuki, Takashi ; Komune, Noritaka ; Noda, Teppei ; Takaiwa, Kazutaka ; Ohashi, Mitsuru ; Komune, Shizuo. / Property of IK,n in inner hair cells isolated from guinea-pig cochlea. In: Hearing Research. 2010 ; Vol. 261, No. 1-2. pp. 57-62.
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AU - Ohashi, Mitsuru

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