Abstract
Propionibacterium acnes (P. acnes) causes an inflammatory acne that is characterized by massive neutrophilic infiltration. IL-8 is thought to play an important role in the pathophysiology of P. acnes, although the mechanisms by which P. acnes up-regulates the release of IL-8, a neutrophilic chemokine, from target cells is not well understood. In this study, we investigated the mechanisms through which heat-killed P. acnes induces IL-8 production in THP-1 cells (a human monocytic cell line). We found that P. acnes is able to directly induce IL-8 production and IL-8 mRNA expression in human monocytic cells in a dose- and time-dependent manner through a mechanism requiring transcription factor NF-κB activation. Additionally, P. acnes-induced IL-8 secretion was inhibited by roxithromycin, a macrolide antibiotic, and its inhibitory effect seemed to be partially associated with the inhibition of P. acnes-induced NF-κB activation. This is the first study to show that NF-κB activation is involved in the IL-8 production of monocytic cells stimulated by P. acnes.
| Original language | English |
|---|---|
| Pages (from-to) | 97-103 |
| Number of pages | 7 |
| Journal | Journal of Dermatological Science |
| Volume | 29 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Jul 2 2002 |
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All Science Journal Classification (ASJC) codes
- Biochemistry
- Molecular Biology
- Dermatology
Cite this
Propionibacterium acnes-induced IL-8 production may be mediated by NF-κB activation in human monocytes. / Chen, Qi Jie; Koga, Tetsuya; Uchi, Hiroshi; Hara, Hiromitsu; Terao, Hiroshi; Moroi, Yoichi; Urabe, Kazunori; Furue, Masutaka.
In: Journal of Dermatological Science, Vol. 29, No. 2, 02.07.2002, p. 97-103.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Propionibacterium acnes-induced IL-8 production may be mediated by NF-κB activation in human monocytes
AU - Chen, Qi Jie
AU - Koga, Tetsuya
AU - Uchi, Hiroshi
AU - Hara, Hiromitsu
AU - Terao, Hiroshi
AU - Moroi, Yoichi
AU - Urabe, Kazunori
AU - Furue, Masutaka
PY - 2002/7/2
Y1 - 2002/7/2
N2 - Propionibacterium acnes (P. acnes) causes an inflammatory acne that is characterized by massive neutrophilic infiltration. IL-8 is thought to play an important role in the pathophysiology of P. acnes, although the mechanisms by which P. acnes up-regulates the release of IL-8, a neutrophilic chemokine, from target cells is not well understood. In this study, we investigated the mechanisms through which heat-killed P. acnes induces IL-8 production in THP-1 cells (a human monocytic cell line). We found that P. acnes is able to directly induce IL-8 production and IL-8 mRNA expression in human monocytic cells in a dose- and time-dependent manner through a mechanism requiring transcription factor NF-κB activation. Additionally, P. acnes-induced IL-8 secretion was inhibited by roxithromycin, a macrolide antibiotic, and its inhibitory effect seemed to be partially associated with the inhibition of P. acnes-induced NF-κB activation. This is the first study to show that NF-κB activation is involved in the IL-8 production of monocytic cells stimulated by P. acnes.
AB - Propionibacterium acnes (P. acnes) causes an inflammatory acne that is characterized by massive neutrophilic infiltration. IL-8 is thought to play an important role in the pathophysiology of P. acnes, although the mechanisms by which P. acnes up-regulates the release of IL-8, a neutrophilic chemokine, from target cells is not well understood. In this study, we investigated the mechanisms through which heat-killed P. acnes induces IL-8 production in THP-1 cells (a human monocytic cell line). We found that P. acnes is able to directly induce IL-8 production and IL-8 mRNA expression in human monocytic cells in a dose- and time-dependent manner through a mechanism requiring transcription factor NF-κB activation. Additionally, P. acnes-induced IL-8 secretion was inhibited by roxithromycin, a macrolide antibiotic, and its inhibitory effect seemed to be partially associated with the inhibition of P. acnes-induced NF-κB activation. This is the first study to show that NF-κB activation is involved in the IL-8 production of monocytic cells stimulated by P. acnes.
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UR - http://www.scopus.com/inward/citedby.url?scp=0036092586&partnerID=8YFLogxK
U2 - 10.1016/S0923-1811(02)00013-0
DO - 10.1016/S0923-1811(02)00013-0
M3 - Article
C2 - 12088610
AN - SCOPUS:0036092586
VL - 29
SP - 97
EP - 103
JO - Journal of Dermatological Science
JF - Journal of Dermatological Science
SN - 0923-1811
IS - 2
ER -