Propofol anaesthesia in mice is potentiated by muscimol and reversed by bicuculline

M. Irifune; M. Sugimura; T. Takarada; K. Maeoka; Y. Shimizu; T. Dohi; T. Nishikawa; M. Kawahara

doi:10.1093/bja/83.4.665

Propofol anaesthesia in mice is potentiated by muscimol and reversed by bicuculline

M. Irifune, M. Sugimura, T. Takarada, K. Maeoka, Y. Shimizu, T. Dohi, T. Nishikawa, M. Kawahara

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

We have examined the role of γ-aminobutyric acid (GABA) neurones in propofol anaesthesia in mice using the righting reflex. Propofol i.p. increased the percentage of loss of the righting reflex in a dose-dependent manner with an ED₅₀ value of 140 (95% confidence limits 123-160) mg kg^-1 (n = 40; eight animals per dose, five doses per dose-response curve). The ED₅₀ for propofol decreased significantly to 66 (58-75) mg kg^-1 in the presence of the GABA(A) receptor agonist muscimol mg kg^-1 i.p. (n = 40) (P < 0.05). In contrast, the ED₅₀ increased significantly to 240 (211-274) mg kg^-1 in the presence of the antagonist bicuculline 5 mg kg^-1 i.p. (n = 40) (P < 0.05). Our results suggest that propofol anaesthesia may be mediated, at least in part, by GABA neurones.

Original language	English
Pages (from-to)	665-667
Number of pages	3
Journal	British Journal of Anaesthesia
Volume	83
Issue number	4
DOIs	https://doi.org/10.1093/bja/83.4.665
Publication status	Published - Oct 1999
Externally published	Yes

All Science Journal Classification (ASJC) codes

Anesthesiology and Pain Medicine

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title = "Propofol anaesthesia in mice is potentiated by muscimol and reversed by bicuculline",

abstract = "We have examined the role of γ-aminobutyric acid (GABA) neurones in propofol anaesthesia in mice using the righting reflex. Propofol i.p. increased the percentage of loss of the righting reflex in a dose-dependent manner with an ED50 value of 140 (95% confidence limits 123-160) mg kg-1 (n = 40; eight animals per dose, five doses per dose-response curve). The ED50 for propofol decreased significantly to 66 (58-75) mg kg-1 in the presence of the GABA(A) receptor agonist muscimol mg kg-1 i.p. (n = 40) (P < 0.05). In contrast, the ED50 increased significantly to 240 (211-274) mg kg-1 in the presence of the antagonist bicuculline 5 mg kg-1 i.p. (n = 40) (P < 0.05). Our results suggest that propofol anaesthesia may be mediated, at least in part, by GABA neurones.",

author = "M. Irifune and M. Sugimura and T. Takarada and K. Maeoka and Y. Shimizu and T. Dohi and T. Nishikawa and M. Kawahara",

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AU - Irifune, M.

AU - Sugimura, M.

AU - Takarada, T.

AU - Maeoka, K.

AU - Shimizu, Y.

AU - Dohi, T.

AU - Nishikawa, T.

AU - Kawahara, M.

PY - 1999/10

Y1 - 1999/10

N2 - We have examined the role of γ-aminobutyric acid (GABA) neurones in propofol anaesthesia in mice using the righting reflex. Propofol i.p. increased the percentage of loss of the righting reflex in a dose-dependent manner with an ED50 value of 140 (95% confidence limits 123-160) mg kg-1 (n = 40; eight animals per dose, five doses per dose-response curve). The ED50 for propofol decreased significantly to 66 (58-75) mg kg-1 in the presence of the GABA(A) receptor agonist muscimol mg kg-1 i.p. (n = 40) (P < 0.05). In contrast, the ED50 increased significantly to 240 (211-274) mg kg-1 in the presence of the antagonist bicuculline 5 mg kg-1 i.p. (n = 40) (P < 0.05). Our results suggest that propofol anaesthesia may be mediated, at least in part, by GABA neurones.

AB - We have examined the role of γ-aminobutyric acid (GABA) neurones in propofol anaesthesia in mice using the righting reflex. Propofol i.p. increased the percentage of loss of the righting reflex in a dose-dependent manner with an ED50 value of 140 (95% confidence limits 123-160) mg kg-1 (n = 40; eight animals per dose, five doses per dose-response curve). The ED50 for propofol decreased significantly to 66 (58-75) mg kg-1 in the presence of the GABA(A) receptor agonist muscimol mg kg-1 i.p. (n = 40) (P < 0.05). In contrast, the ED50 increased significantly to 240 (211-274) mg kg-1 in the presence of the antagonist bicuculline 5 mg kg-1 i.p. (n = 40) (P < 0.05). Our results suggest that propofol anaesthesia may be mediated, at least in part, by GABA neurones.

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