TY - JOUR
T1 - Propofol anaesthesia in mice is potentiated by muscimol and reversed by bicuculline
AU - Irifune, M.
AU - Sugimura, M.
AU - Takarada, T.
AU - Maeoka, K.
AU - Shimizu, Y.
AU - Dohi, T.
AU - Nishikawa, T.
AU - Kawahara, M.
PY - 1999/10
Y1 - 1999/10
N2 - We have examined the role of γ-aminobutyric acid (GABA) neurones in propofol anaesthesia in mice using the righting reflex. Propofol i.p. increased the percentage of loss of the righting reflex in a dose-dependent manner with an ED50 value of 140 (95% confidence limits 123-160) mg kg-1 (n = 40; eight animals per dose, five doses per dose-response curve). The ED50 for propofol decreased significantly to 66 (58-75) mg kg-1 in the presence of the GABA(A) receptor agonist muscimol mg kg-1 i.p. (n = 40) (P < 0.05). In contrast, the ED50 increased significantly to 240 (211-274) mg kg-1 in the presence of the antagonist bicuculline 5 mg kg-1 i.p. (n = 40) (P < 0.05). Our results suggest that propofol anaesthesia may be mediated, at least in part, by GABA neurones.
AB - We have examined the role of γ-aminobutyric acid (GABA) neurones in propofol anaesthesia in mice using the righting reflex. Propofol i.p. increased the percentage of loss of the righting reflex in a dose-dependent manner with an ED50 value of 140 (95% confidence limits 123-160) mg kg-1 (n = 40; eight animals per dose, five doses per dose-response curve). The ED50 for propofol decreased significantly to 66 (58-75) mg kg-1 in the presence of the GABA(A) receptor agonist muscimol mg kg-1 i.p. (n = 40) (P < 0.05). In contrast, the ED50 increased significantly to 240 (211-274) mg kg-1 in the presence of the antagonist bicuculline 5 mg kg-1 i.p. (n = 40) (P < 0.05). Our results suggest that propofol anaesthesia may be mediated, at least in part, by GABA neurones.
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U2 - 10.1093/bja/83.4.665
DO - 10.1093/bja/83.4.665
M3 - Article
C2 - 10673890
AN - SCOPUS:0032882424
VL - 83
SP - 665
EP - 667
JO - British Journal of Anaesthesia
JF - British Journal of Anaesthesia
SN - 0007-0912
IS - 4
ER -