Abstract
We examined the effects of a general anesthetic 2,6-diisopropylphenol (propofol) on ATP- and α,β-methylene ATP (αβmeATP)-activated currents in the human embryonic kidney 293 (HEK 293) cells expressing recombinant P2X receptor channels, using the whole-cell patch-clamp method. Propofol at clinical relevant concentrations (~56 μM) potentiated the current responses through the P2X4 receptor in a dose-dependent manner, whereas propofol did not affect the responses through the P2X2 receptor or through the heterologous complex of the P2X2 and P2X3 (P2X2+3) receptor. These results suggest that activation of P2X4 subtype in the brain and the motor neurons of the spinal anterior horn might be involved in the excitatory effect by propofol such as convulsion and unexpected movements. Copyright (C) 2000 Elsevier Science Ireland Ltd.
| Original language | English |
|---|---|
| Pages (from-to) | 167-170 |
| Number of pages | 4 |
| Journal | Neuroscience Letters |
| Volume | 284 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Apr 28 2000 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Neuroscience(all)
Cite this
Propofol potentiates ATP-activated currents of recombinant P2X4 receptor channels expressed in human embryonic kidney 293 cells. / Tomioka, Akihiro; Ueno, Shinya; Kohama, Kazuhiro; Goto, Fumio; Inoue, Kazuhide.
In: Neuroscience Letters, Vol. 284, No. 3, 28.04.2000, p. 167-170.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Propofol potentiates ATP-activated currents of recombinant P2X4 receptor channels expressed in human embryonic kidney 293 cells
AU - Tomioka, Akihiro
AU - Ueno, Shinya
AU - Kohama, Kazuhiro
AU - Goto, Fumio
AU - Inoue, Kazuhide
PY - 2000/4/28
Y1 - 2000/4/28
N2 - We examined the effects of a general anesthetic 2,6-diisopropylphenol (propofol) on ATP- and α,β-methylene ATP (αβmeATP)-activated currents in the human embryonic kidney 293 (HEK 293) cells expressing recombinant P2X receptor channels, using the whole-cell patch-clamp method. Propofol at clinical relevant concentrations (~56 μM) potentiated the current responses through the P2X4 receptor in a dose-dependent manner, whereas propofol did not affect the responses through the P2X2 receptor or through the heterologous complex of the P2X2 and P2X3 (P2X2+3) receptor. These results suggest that activation of P2X4 subtype in the brain and the motor neurons of the spinal anterior horn might be involved in the excitatory effect by propofol such as convulsion and unexpected movements. Copyright (C) 2000 Elsevier Science Ireland Ltd.
AB - We examined the effects of a general anesthetic 2,6-diisopropylphenol (propofol) on ATP- and α,β-methylene ATP (αβmeATP)-activated currents in the human embryonic kidney 293 (HEK 293) cells expressing recombinant P2X receptor channels, using the whole-cell patch-clamp method. Propofol at clinical relevant concentrations (~56 μM) potentiated the current responses through the P2X4 receptor in a dose-dependent manner, whereas propofol did not affect the responses through the P2X2 receptor or through the heterologous complex of the P2X2 and P2X3 (P2X2+3) receptor. These results suggest that activation of P2X4 subtype in the brain and the motor neurons of the spinal anterior horn might be involved in the excitatory effect by propofol such as convulsion and unexpected movements. Copyright (C) 2000 Elsevier Science Ireland Ltd.
UR - http://www.scopus.com/inward/record.url?scp=0034724976&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034724976&partnerID=8YFLogxK
U2 - 10.1016/S0304-3940(00)01014-4
DO - 10.1016/S0304-3940(00)01014-4
M3 - Article
C2 - 10773425
AN - SCOPUS:0034724976
VL - 284
SP - 167
EP - 170
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
IS - 3
ER -