Propofol potentiates ATP-activated currents of recombinant P2X4 receptor channels expressed in human embryonic kidney 293 cells

Akihiro Tomioka, Shinya Ueno, Kazuhiro Kohama, Fumio Goto, Kazuhide Inoue

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

We examined the effects of a general anesthetic 2,6-diisopropylphenol (propofol) on ATP- and α,β-methylene ATP (αβmeATP)-activated currents in the human embryonic kidney 293 (HEK 293) cells expressing recombinant P2X receptor channels, using the whole-cell patch-clamp method. Propofol at clinical relevant concentrations (~56 μM) potentiated the current responses through the P2X4 receptor in a dose-dependent manner, whereas propofol did not affect the responses through the P2X2 receptor or through the heterologous complex of the P2X2 and P2X3 (P2X2+3) receptor. These results suggest that activation of P2X4 subtype in the brain and the motor neurons of the spinal anterior horn might be involved in the excitatory effect by propofol such as convulsion and unexpected movements. Copyright (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)167-170
Number of pages4
JournalNeuroscience Letters
Volume284
Issue number3
DOIs
Publication statusPublished - Apr 28 2000
Externally publishedYes

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Purinergic P2X4 Receptors
Propofol
Adenosine Triphosphate
Kidney
Purinergic P2X2 Receptors
General Anesthetics
Motor Neurons
Horns
Seizures
Brain

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Propofol potentiates ATP-activated currents of recombinant P2X4 receptor channels expressed in human embryonic kidney 293 cells. / Tomioka, Akihiro; Ueno, Shinya; Kohama, Kazuhiro; Goto, Fumio; Inoue, Kazuhide.

In: Neuroscience Letters, Vol. 284, No. 3, 28.04.2000, p. 167-170.

Research output: Contribution to journalArticle

Tomioka, Akihiro ; Ueno, Shinya ; Kohama, Kazuhiro ; Goto, Fumio ; Inoue, Kazuhide. / Propofol potentiates ATP-activated currents of recombinant P2X4 receptor channels expressed in human embryonic kidney 293 cells. In: Neuroscience Letters. 2000 ; Vol. 284, No. 3. pp. 167-170.
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