Propofol prevents mitochondrial fragmentation

Morika Suzuki, Hiroyuki Uchino, Hiroki Kato

Research output: Contribution to journalArticle

Abstract

Background : Propofol is a short-acting analgesic agent frequently used for intravenous anesthesia. In addition to having an anesthetic function, it has recently been reported to exert a neuro-protective effect during cerebral ischemia reperfusion. Although it has been suggested that propofol suppresses apoptosis in neuronal cells induced by reperfusion of ischemic tissue, much of the neuro-protective mechanism of propofol remains to be clarified. The purpose of this study was to investigate whether propofol affected mitochondrial fragmentation caused by mitochondrial fission, the initial stage of apoptosis. Methods : Human neuroblastoma SH-SY5Y cells were treated with CCCP, an uncoupler, in the presence or absence of propofol. Fragmentation of mitochondria was evaluated by live cell imaging ; cleavage of OPA1, a mitochondrial fusion factor cleaved and generated as a short form (S-OPAl) from a long form (L-OPAl) during mitochondrial fission, was detected by western blotting. Results: Mitochondrial fragmentation induced by CCCP was suppressed by propofol. Moreover, cleavage of L-OPAl to S-OPAl was delayed by the addition of propofol. Conclusion : The present results showed that propofol delayed the fragmentation of mitochondria. This suggests that the neuro-protective function of propofol is due to delayed mitochondrial fragmentation during apoptosis.

Original languageEnglish
Pages (from-to)268-275
Number of pages8
JournalJournal of Tokyo Medical University
Volume73
Issue number3
Publication statusPublished - Jan 1 2015

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Propofol
Mitochondrial Dynamics
Carbonyl Cyanide m-Chlorophenyl Hydrazone
Apoptosis
Short-Acting Analgesics
Reperfusion
Mitochondria
L Forms
Intravenous Anesthesia
Brain Ischemia
Neuroblastoma
Analgesics
Anesthetics
Western Blotting

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Propofol prevents mitochondrial fragmentation. / Suzuki, Morika; Uchino, Hiroyuki; Kato, Hiroki.

In: Journal of Tokyo Medical University, Vol. 73, No. 3, 01.01.2015, p. 268-275.

Research output: Contribution to journalArticle

Suzuki, M, Uchino, H & Kato, H 2015, 'Propofol prevents mitochondrial fragmentation', Journal of Tokyo Medical University, vol. 73, no. 3, pp. 268-275.
Suzuki, Morika ; Uchino, Hiroyuki ; Kato, Hiroki. / Propofol prevents mitochondrial fragmentation. In: Journal of Tokyo Medical University. 2015 ; Vol. 73, No. 3. pp. 268-275.
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