Proposal of new modification technique for linear double-stranded DNAs using the polysaccharide schizopyllan

Takahisa Anada, Hideshi Matsunaga, Ryouji Karinaga, Kazuya Koumoto, Masami Mizu, Koji Nakano, Seiji Shinkai, Kazuo Sakurai

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Abstract

A poly(dA) tailed double-stranded DNA and its polysaccharide complexes are reported. A natural polysaccharide schizophyllan (SPG) has been known to form a stable complex with poly(dA). We attached a poly(dA) 80 tail to the both ends of a linear double-stranded DNA, which had been prepared from a plasmid DNA vector. The poly(dA) tailed DNA verified to form complex with SPG by gel electrophoresis and atomic force microscopy (AFM). AFM images indicated that the complexes exhibit a dumbbell-like architecture, that is, quite similar to that of adenovirus genome. The complex demonstrated excellent exonuclease resistance, probably because of the protection effect by SPG complexation.

Original languageEnglish
Pages (from-to)5655-5659
Number of pages5
JournalBioorganic and Medicinal Chemistry Letters
Volume14
Issue number22
DOIs
Publication statusPublished - Nov 15 2004

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All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

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