TY - JOUR
T1 - Proposal of new modification technique for linear double-stranded DNAs using the polysaccharide schizopyllan
AU - Anada, Takahisa
AU - Matsunaga, Hideshi
AU - Karinaga, Ryouji
AU - Koumoto, Kazuya
AU - Mizu, Masami
AU - Nakano, Koji
AU - Shinkai, Seiji
AU - Sakurai, Kazuo
N1 - Funding Information:
This work is financially supported by the SORST program of Japan Science and Technology Agency (JST).
Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2004/11/15
Y1 - 2004/11/15
N2 - A poly(dA) tailed double-stranded DNA and its polysaccharide complexes are reported. A natural polysaccharide schizophyllan (SPG) has been known to form a stable complex with poly(dA). We attached a poly(dA) 80 tail to the both ends of a linear double-stranded DNA, which had been prepared from a plasmid DNA vector. The poly(dA) tailed DNA verified to form complex with SPG by gel electrophoresis and atomic force microscopy (AFM). AFM images indicated that the complexes exhibit a dumbbell-like architecture, that is, quite similar to that of adenovirus genome. The complex demonstrated excellent exonuclease resistance, probably because of the protection effect by SPG complexation.
AB - A poly(dA) tailed double-stranded DNA and its polysaccharide complexes are reported. A natural polysaccharide schizophyllan (SPG) has been known to form a stable complex with poly(dA). We attached a poly(dA) 80 tail to the both ends of a linear double-stranded DNA, which had been prepared from a plasmid DNA vector. The poly(dA) tailed DNA verified to form complex with SPG by gel electrophoresis and atomic force microscopy (AFM). AFM images indicated that the complexes exhibit a dumbbell-like architecture, that is, quite similar to that of adenovirus genome. The complex demonstrated excellent exonuclease resistance, probably because of the protection effect by SPG complexation.
UR - http://www.scopus.com/inward/record.url?scp=5144223002&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=5144223002&partnerID=8YFLogxK
U2 - 10.1016/j.bmcl.2004.08.043
DO - 10.1016/j.bmcl.2004.08.043
M3 - Article
C2 - 15482942
AN - SCOPUS:5144223002
SN - 0960-894X
VL - 14
SP - 5655
EP - 5659
JO - Bioorganic and Medicinal Chemistry Letters
JF - Bioorganic and Medicinal Chemistry Letters
IS - 22
ER -