Prostaglandin E2-prostoglandin E receptor subtype 4 (EP4) signaling mediates UV irradiation-induced systemic immunosuppression

Kitipong Soontrapa, Tetsuya Honda, Daiji Sakata, Chengcan Yao, Takako Hirata, Shohei Hori, Toshiyuki Matsuoka, Yoshihiro Kita, Takao Shimizu, Kenji Kabashima, Shuh Narumiya

Research output: Contribution to journalArticle

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Abstract

UV radiation induces systemic immunosuppression. Because nonsteroidal anti-inflammatory drugs suppress UV-induced immunosuppression, prostanoids have been suspected as a crucial mediator of this UV effect. However, the identity of the prostanoid involved and its mechanism of action remain unclear. Here, we addressed this issue by subjecting mice deficient in each prostanoid receptor individually or mice treated with a subtype-specific antagonist to UV irradiation. Mice treated with an antagonist for prostaglandin E receptor subtype 4 (EP4), but not those deficient in other prostanoid receptors, show impaired UV-induced immunosuppression, whereas administration of an EP4 agonist rescues the impairment of the UV-induced immunosuppression in indomethacin-treated mice. The EP4 antagonist treatment suppresses an increase in the number of CD4+/forkhead box P3-positive (Foxp3+) regulatory T cells (Treg cells) in the peripheral lymph nodes (LNs) and dendritic cells expressing DEC205 in the LNs and the skin after UV irradiation. Furthermore, the EP4 antagonist treatment down-regulates UV-induced expression of receptor activator of NF-κB ligand (RANKL) in skin keratinocytes. Finally, administration of anti-RANKL antibody abolishes the restoration of UV-induced immunosuppression by EP4 agonism in indomethacin-treated mice. Thus, prostaglandin E2 (PGE2)-EP4 signaling mediates UV-induced immunosuppression by elevating the number of Treg cells through regulation of RANKL expression in the epidermis.

Original languageEnglish
Pages (from-to)6668-6673
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume108
Issue number16
DOIs
Publication statusPublished - Apr 19 2011

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Dinoprostone
Immunosuppression
Prostaglandins
Regulatory T-Lymphocytes
Indomethacin
Receptors, Prostaglandin E, EP4 Subtype
Lymph Nodes
Skin
Keratinocytes
Epidermis
Dendritic Cells
Anti-Idiotypic Antibodies
Anti-Inflammatory Agents
Down-Regulation
Radiation
Ligands
Therapeutics
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • General

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Prostaglandin E2-prostoglandin E receptor subtype 4 (EP4) signaling mediates UV irradiation-induced systemic immunosuppression. / Soontrapa, Kitipong; Honda, Tetsuya; Sakata, Daiji; Yao, Chengcan; Hirata, Takako; Hori, Shohei; Matsuoka, Toshiyuki; Kita, Yoshihiro; Shimizu, Takao; Kabashima, Kenji; Narumiya, Shuh.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, No. 16, 19.04.2011, p. 6668-6673.

Research output: Contribution to journalArticle

Soontrapa, Kitipong ; Honda, Tetsuya ; Sakata, Daiji ; Yao, Chengcan ; Hirata, Takako ; Hori, Shohei ; Matsuoka, Toshiyuki ; Kita, Yoshihiro ; Shimizu, Takao ; Kabashima, Kenji ; Narumiya, Shuh. / Prostaglandin E2-prostoglandin E receptor subtype 4 (EP4) signaling mediates UV irradiation-induced systemic immunosuppression. In: Proceedings of the National Academy of Sciences of the United States of America. 2011 ; Vol. 108, No. 16. pp. 6668-6673.
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