Prostaglandin E2 attenuates preoptic expression of GABA A receptors via EP3 receptors

Hiroyoshi Tsuchiya, Takakazu Oka, Kazuhiro Nakamura, Atsushi Ichikawa, Clifford B. Saper, Yukihiko Sugimoto

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Prostaglandin E2 (PGE2) has been shown to produce fever by acting on EP3 receptors within the preoptic area of the brain. However, there is little information about the molecular events downstream of EP3 activation in preoptic neurons. As a first step toward this issue, we examined PGE2-induced gene expression changes at single-cell resolution in preoptic neurons expressing EP3. Brain sections of the preoptic area from PGE2-or saline-injected rats were stained with an anti-EP3 antibody, and the cell bodies of EP3-positive neurons were dissected and subjected to RNA amplification procedures. Microarray analysis of the amplified products demonstrated the possibility that gene expression of γ-aminobutyric acid type A (GABAA) receptor subunits is decreased upon PGE2 injection. Indeed, we found that most EP3-positive neurons in the mouse preoptic area are positive for the α2 or γ2 GABAA receptor subunit. Moreover, PGE2 decreased the preoptic gene expression of these GABAA subunits via an EP3-dependent and pertussis toxin-sensitive pathway. PGE2 also attenuated the preoptic protein expression of the α2 subunit in wild-type but not in EP3-deficient mice. These results indicate that PGE2-EP3 signaling elicits G i/oactivation in preoptic thermocenter neurons, and we propose the possibility that a rapid decrease in preoptic GABAA expression may be involved in PGE2-induced fever.

Original languageEnglish
Pages (from-to)11064-11071
Number of pages8
JournalJournal of Biological Chemistry
Volume283
Issue number16
DOIs
Publication statusPublished - Apr 18 2008

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GABA-A Receptors
Dinoprostone
Gene expression
Aminobutyrates
Neurons
Gene Expression
Preoptic Area
Brain
Fever
Pertussis Toxin
Microarray Analysis
Microarrays
Amplification
Rats
Anti-Idiotypic Antibodies
Chemical activation
Cells
RNA
Injections
Antibodies

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Tsuchiya, H., Oka, T., Nakamura, K., Ichikawa, A., Saper, C. B., & Sugimoto, Y. (2008). Prostaglandin E2 attenuates preoptic expression of GABA A receptors via EP3 receptors. Journal of Biological Chemistry, 283(16), 11064-11071. https://doi.org/10.1074/jbc.M801359200

Prostaglandin E2 attenuates preoptic expression of GABA A receptors via EP3 receptors. / Tsuchiya, Hiroyoshi; Oka, Takakazu; Nakamura, Kazuhiro; Ichikawa, Atsushi; Saper, Clifford B.; Sugimoto, Yukihiko.

In: Journal of Biological Chemistry, Vol. 283, No. 16, 18.04.2008, p. 11064-11071.

Research output: Contribution to journalArticle

Tsuchiya, H, Oka, T, Nakamura, K, Ichikawa, A, Saper, CB & Sugimoto, Y 2008, 'Prostaglandin E2 attenuates preoptic expression of GABA A receptors via EP3 receptors', Journal of Biological Chemistry, vol. 283, no. 16, pp. 11064-11071. https://doi.org/10.1074/jbc.M801359200
Tsuchiya, Hiroyoshi ; Oka, Takakazu ; Nakamura, Kazuhiro ; Ichikawa, Atsushi ; Saper, Clifford B. ; Sugimoto, Yukihiko. / Prostaglandin E2 attenuates preoptic expression of GABA A receptors via EP3 receptors. In: Journal of Biological Chemistry. 2008 ; Vol. 283, No. 16. pp. 11064-11071.
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