TY - JOUR
T1 - Prostaglandin E2 may induce hyperthermia through EP1 receptor in the anterior wall of the third ventricle and neighboring preoptic regions
AU - Oka, Kae
AU - Oka, Takakazu
AU - Hori, Tetsuro
N1 - Funding Information:
This study was supported in part by Grants-in-Aid for Scientific Research Nos. 06454153 and 06557006 (to T.H.) and grants from the Naito Foundation 93-183 (to T.H.) and 95-185 (to T.O.). This experiment was reviewed by the Committee of Ethics on Animal Experiments in the Faculty of Medicine, Kyushu University and was carried out under the control of the Guidelines for Animal Experiments of the Faculty of Medicine, Kyushu University and the Law (No. 105) and Notification (No. 6) of the Government.
PY - 1997/8/29
Y1 - 1997/8/29
N2 - We have previously reported that intracerebroventricular injection of prostaglandin E2 (PGE2) induces hyperthermia possibly through EP1 receptors in the rat. In the present study, to determine the sites in the brain where PGE2 induces hyperthermia through EP1 receptors, we microinjected an EP1 receptor agonist, 17-phenyl-ω-trinor PGE2 (17-Ph- PGE2, 100 ng) into different sites in the rat brain and observed the colonic temperature (T(co)) for 2 h in a 23 ± 1°C environment. Responsive sites where 17-Ph-PGE2 (100 ng) produced a rise in the T(co) of more than 1.1°C within 60 min after injection were found in the medial preoptic area, the subchiasmatic portion of the median preoptic nucleus, the anterior wail of the third ventricle (A3V) and the ventral portion of the diagonal band of Broca. Among these sites, the A3V was the most responsive. In contrast, microinjection of neither butaprost (an EP2 agonist, 100 ng) nor M and B28767 (an EP3 agonist, 100 ng) into these four sites had any effect on the T(co). Intracerebroventricular pretreatment with SC-19220 (an EP1 antagonist, 100 μg) inhibited the rise in the T(co) which was induced by microinjection of PGE2 (50 ng) into the A3V. These results thus suggest that PGE2 induces hyperthermia by stimulating EP1 receptors in the A3V and the neighboring preoptic region.
AB - We have previously reported that intracerebroventricular injection of prostaglandin E2 (PGE2) induces hyperthermia possibly through EP1 receptors in the rat. In the present study, to determine the sites in the brain where PGE2 induces hyperthermia through EP1 receptors, we microinjected an EP1 receptor agonist, 17-phenyl-ω-trinor PGE2 (17-Ph- PGE2, 100 ng) into different sites in the rat brain and observed the colonic temperature (T(co)) for 2 h in a 23 ± 1°C environment. Responsive sites where 17-Ph-PGE2 (100 ng) produced a rise in the T(co) of more than 1.1°C within 60 min after injection were found in the medial preoptic area, the subchiasmatic portion of the median preoptic nucleus, the anterior wail of the third ventricle (A3V) and the ventral portion of the diagonal band of Broca. Among these sites, the A3V was the most responsive. In contrast, microinjection of neither butaprost (an EP2 agonist, 100 ng) nor M and B28767 (an EP3 agonist, 100 ng) into these four sites had any effect on the T(co). Intracerebroventricular pretreatment with SC-19220 (an EP1 antagonist, 100 μg) inhibited the rise in the T(co) which was induced by microinjection of PGE2 (50 ng) into the A3V. These results thus suggest that PGE2 induces hyperthermia by stimulating EP1 receptors in the A3V and the neighboring preoptic region.
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U2 - 10.1016/S0006-8993(97)00562-3
DO - 10.1016/S0006-8993(97)00562-3
M3 - Article
C2 - 9365020
AN - SCOPUS:0030613623
SN - 0006-8993
VL - 767
SP - 92
EP - 99
JO - Molecular Brain Research
JF - Molecular Brain Research
IS - 1
ER -