TY - JOUR
T1 - Prostaglandin E2 receptor Ptger4b regulates female-specific peptidergic neurons and female sexual receptivity in medaka
AU - Fleming, Thomas
AU - Kikuchi, Yukiko
AU - Nakajo, Mikoto
AU - Tachizawa, Masaya
AU - Inazumi, Tomoaki
AU - Tsuchiya, Soken
AU - Sugimoto, Yukihiko
AU - Saito, Daisuke
AU - Suyama, Mikita
AU - Ohkawa, Yasuyuki
AU - Baba, Takashi
AU - Morohashi, Ken ichirou
AU - Okubo, Kataaki
N1 - Funding Information:
We thank National BioResource Project (NBRP) Medaka for providing the fosmid clone used in this study. We also thank Dr. Hideki Abe for technical advice on isolation and purification of FeSP neurons; Dr. Junpei Yamashita for performing ovariectomy; Dr. Yoshitaka Oka for useful discussions and providing the equipment for patch-clamp recordings; Yuji Nishiike and Kaito Ishikawa for overall experimental support; and Akira Hirata, Tomiko Iba, Kaoru Furukawa, and Ayu Kuwakubo for assistance with medaka husbandry. This work was supported by the Ministry of Education, Culture, Sports, Science, and Technology (MEXT) in Japan and the Japan Society for the Promotion of Science (JSPS) (MEXT/JSPS grant numbers 20J13802 (to TF), 16J03203 (to YK), 16H04979, 17H06429, and 19H03044 (to KO)).
Publisher Copyright:
© 2022, The Author(s).
PY - 2022/12
Y1 - 2022/12
N2 - In vertebrates, female receptivity to male courtship is highly dependent on ovarian secretion of estrogens and prostaglandins. We recently identified female-specific neurons in the medaka (Oryzias latipes) preoptic area that express Npba, a neuropeptide mediating female sexual receptivity, in response to ovarian estrogens. Here we show by transcriptomic analysis that these neurons express a multitude of neuropeptides, in addition to Npba, in an ovarian-dependent manner, and we thus termed them female-specific, sex steroid-responsive peptidergic (FeSP) neurons. Our results further revealed that FeSP neurons express a prostaglandin E2 receptor gene, ptger4b, in an ovarian estrogen-dependent manner. Behavioral and physiological examination of ptger4b-deficient female medaka found that they exhibit increased sexual receptivity while retaining normal ovarian function and that their FeSP neurons have reduced firing activity and impaired neuropeptide release. Collectively, this work provides evidence that prostaglandin E2/Ptger4b signaling mediates the estrogenic regulation of FeSP neuron activity and female sexual receptivity.
AB - In vertebrates, female receptivity to male courtship is highly dependent on ovarian secretion of estrogens and prostaglandins. We recently identified female-specific neurons in the medaka (Oryzias latipes) preoptic area that express Npba, a neuropeptide mediating female sexual receptivity, in response to ovarian estrogens. Here we show by transcriptomic analysis that these neurons express a multitude of neuropeptides, in addition to Npba, in an ovarian-dependent manner, and we thus termed them female-specific, sex steroid-responsive peptidergic (FeSP) neurons. Our results further revealed that FeSP neurons express a prostaglandin E2 receptor gene, ptger4b, in an ovarian estrogen-dependent manner. Behavioral and physiological examination of ptger4b-deficient female medaka found that they exhibit increased sexual receptivity while retaining normal ovarian function and that their FeSP neurons have reduced firing activity and impaired neuropeptide release. Collectively, this work provides evidence that prostaglandin E2/Ptger4b signaling mediates the estrogenic regulation of FeSP neuron activity and female sexual receptivity.
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U2 - 10.1038/s42003-022-04195-x
DO - 10.1038/s42003-022-04195-x
M3 - Article
C2 - 36357668
AN - SCOPUS:85141604775
VL - 5
JO - Communications Biology
JF - Communications Biology
SN - 2399-3642
IS - 1
M1 - 1215
ER -