Prostate expression of endothelins and their receptors in rat growth

Yoshihiro Wada, Wataru Takahashi, Jamshid Latifpour, Robert M. Weiss, Koichiro Matsumoto, Kunihiko Matsui, Gen Yamada, Takahisa Imamura

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Endothelins (ETs) have been shown to have mitogenic effects on prostate cells in vitro. To study a relationship between ETs and prostate growth, we investigated expression of ET-1 and ET-3 and their receptors ETA and ETB in the dorsolateral (DLP) and ventral (VP) prostate lobes of rats aged 3 weeks, 3 months, and 22 months. The weight ratio of each lobe to the body was not different at 3 weeks but increased more in the DLP than in the VP at 3 months; the ratio did not change at 22 months. The density of the major receptor ETA, measured by radio-labelled ET-1 binding to prostate membrane particulates, was highest at 3 weeks in both prostate lobes and decreased thereafter. mRNA expression levels of ETA, ETB, ET-1, and ET-3 in the DLP and ETA in the VP, as assessed by relative multiplex reverse transcription-polymerase chain reaction, were highest at 3 weeks, and no significant change was seen in mRNA expression of ET-converting enzyme-1. The highest expression of ETA and the ligands at 3 weeks, followed by dramatic growth at 3 months in the DLP, suggests a role of ETA-mediated signal transduction in prostate growth of rats and possibly of humans.

Original languageEnglish
Pages (from-to)750-759
Number of pages10
JournalReproduction, Fertility and Development
Volume20
Issue number7
DOIs
Publication statusPublished - Sep 23 2008

Fingerprint

Endothelin Receptors
endothelins
Prostate
receptors
rats
Endothelin-1
Growth
Endothelins
Endothelin-3
Viral Structural Proteins
Messenger RNA
Radio
Reverse Transcription
Signal Transduction
radio
Ligands
signal transduction
Weights and Measures
Polymerase Chain Reaction
particulates

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Reproductive Medicine
  • Animal Science and Zoology
  • Molecular Biology
  • Genetics
  • Endocrinology
  • Developmental Biology

Cite this

Wada, Y., Takahashi, W., Latifpour, J., Weiss, R. M., Matsumoto, K., Matsui, K., ... Imamura, T. (2008). Prostate expression of endothelins and their receptors in rat growth. Reproduction, Fertility and Development, 20(7), 750-759. https://doi.org/10.1071/RD08052

Prostate expression of endothelins and their receptors in rat growth. / Wada, Yoshihiro; Takahashi, Wataru; Latifpour, Jamshid; Weiss, Robert M.; Matsumoto, Koichiro; Matsui, Kunihiko; Yamada, Gen; Imamura, Takahisa.

In: Reproduction, Fertility and Development, Vol. 20, No. 7, 23.09.2008, p. 750-759.

Research output: Contribution to journalArticle

Wada, Y, Takahashi, W, Latifpour, J, Weiss, RM, Matsumoto, K, Matsui, K, Yamada, G & Imamura, T 2008, 'Prostate expression of endothelins and their receptors in rat growth', Reproduction, Fertility and Development, vol. 20, no. 7, pp. 750-759. https://doi.org/10.1071/RD08052
Wada, Yoshihiro ; Takahashi, Wataru ; Latifpour, Jamshid ; Weiss, Robert M. ; Matsumoto, Koichiro ; Matsui, Kunihiko ; Yamada, Gen ; Imamura, Takahisa. / Prostate expression of endothelins and their receptors in rat growth. In: Reproduction, Fertility and Development. 2008 ; Vol. 20, No. 7. pp. 750-759.
@article{64b5ef972913481eaceffb7211f012b8,
title = "Prostate expression of endothelins and their receptors in rat growth",
abstract = "Endothelins (ETs) have been shown to have mitogenic effects on prostate cells in vitro. To study a relationship between ETs and prostate growth, we investigated expression of ET-1 and ET-3 and their receptors ETA and ETB in the dorsolateral (DLP) and ventral (VP) prostate lobes of rats aged 3 weeks, 3 months, and 22 months. The weight ratio of each lobe to the body was not different at 3 weeks but increased more in the DLP than in the VP at 3 months; the ratio did not change at 22 months. The density of the major receptor ETA, measured by radio-labelled ET-1 binding to prostate membrane particulates, was highest at 3 weeks in both prostate lobes and decreased thereafter. mRNA expression levels of ETA, ETB, ET-1, and ET-3 in the DLP and ETA in the VP, as assessed by relative multiplex reverse transcription-polymerase chain reaction, were highest at 3 weeks, and no significant change was seen in mRNA expression of ET-converting enzyme-1. The highest expression of ETA and the ligands at 3 weeks, followed by dramatic growth at 3 months in the DLP, suggests a role of ETA-mediated signal transduction in prostate growth of rats and possibly of humans.",
author = "Yoshihiro Wada and Wataru Takahashi and Jamshid Latifpour and Weiss, {Robert M.} and Koichiro Matsumoto and Kunihiko Matsui and Gen Yamada and Takahisa Imamura",
year = "2008",
month = "9",
day = "23",
doi = "10.1071/RD08052",
language = "English",
volume = "20",
pages = "750--759",
journal = "Reproduction, Fertility and Development",
issn = "1031-3613",
publisher = "CSIRO",
number = "7",

}

TY - JOUR

T1 - Prostate expression of endothelins and their receptors in rat growth

AU - Wada, Yoshihiro

AU - Takahashi, Wataru

AU - Latifpour, Jamshid

AU - Weiss, Robert M.

AU - Matsumoto, Koichiro

AU - Matsui, Kunihiko

AU - Yamada, Gen

AU - Imamura, Takahisa

PY - 2008/9/23

Y1 - 2008/9/23

N2 - Endothelins (ETs) have been shown to have mitogenic effects on prostate cells in vitro. To study a relationship between ETs and prostate growth, we investigated expression of ET-1 and ET-3 and their receptors ETA and ETB in the dorsolateral (DLP) and ventral (VP) prostate lobes of rats aged 3 weeks, 3 months, and 22 months. The weight ratio of each lobe to the body was not different at 3 weeks but increased more in the DLP than in the VP at 3 months; the ratio did not change at 22 months. The density of the major receptor ETA, measured by radio-labelled ET-1 binding to prostate membrane particulates, was highest at 3 weeks in both prostate lobes and decreased thereafter. mRNA expression levels of ETA, ETB, ET-1, and ET-3 in the DLP and ETA in the VP, as assessed by relative multiplex reverse transcription-polymerase chain reaction, were highest at 3 weeks, and no significant change was seen in mRNA expression of ET-converting enzyme-1. The highest expression of ETA and the ligands at 3 weeks, followed by dramatic growth at 3 months in the DLP, suggests a role of ETA-mediated signal transduction in prostate growth of rats and possibly of humans.

AB - Endothelins (ETs) have been shown to have mitogenic effects on prostate cells in vitro. To study a relationship between ETs and prostate growth, we investigated expression of ET-1 and ET-3 and their receptors ETA and ETB in the dorsolateral (DLP) and ventral (VP) prostate lobes of rats aged 3 weeks, 3 months, and 22 months. The weight ratio of each lobe to the body was not different at 3 weeks but increased more in the DLP than in the VP at 3 months; the ratio did not change at 22 months. The density of the major receptor ETA, measured by radio-labelled ET-1 binding to prostate membrane particulates, was highest at 3 weeks in both prostate lobes and decreased thereafter. mRNA expression levels of ETA, ETB, ET-1, and ET-3 in the DLP and ETA in the VP, as assessed by relative multiplex reverse transcription-polymerase chain reaction, were highest at 3 weeks, and no significant change was seen in mRNA expression of ET-converting enzyme-1. The highest expression of ETA and the ligands at 3 weeks, followed by dramatic growth at 3 months in the DLP, suggests a role of ETA-mediated signal transduction in prostate growth of rats and possibly of humans.

UR - http://www.scopus.com/inward/record.url?scp=51949099011&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=51949099011&partnerID=8YFLogxK

U2 - 10.1071/RD08052

DO - 10.1071/RD08052

M3 - Article

VL - 20

SP - 750

EP - 759

JO - Reproduction, Fertility and Development

JF - Reproduction, Fertility and Development

SN - 1031-3613

IS - 7

ER -