Abstract
Endothelins (ETs) have been shown to have mitogenic effects on prostate cells in vitro. To study a relationship between ETs and prostate growth, we investigated expression of ET-1 and ET-3 and their receptors ETA and ETB in the dorsolateral (DLP) and ventral (VP) prostate lobes of rats aged 3 weeks, 3 months, and 22 months. The weight ratio of each lobe to the body was not different at 3 weeks but increased more in the DLP than in the VP at 3 months; the ratio did not change at 22 months. The density of the major receptor ETA, measured by radio-labelled ET-1 binding to prostate membrane particulates, was highest at 3 weeks in both prostate lobes and decreased thereafter. mRNA expression levels of ETA, ETB, ET-1, and ET-3 in the DLP and ETA in the VP, as assessed by relative multiplex reverse transcription-polymerase chain reaction, were highest at 3 weeks, and no significant change was seen in mRNA expression of ET-converting enzyme-1. The highest expression of ETA and the ligands at 3 weeks, followed by dramatic growth at 3 months in the DLP, suggests a role of ETA-mediated signal transduction in prostate growth of rats and possibly of humans.
| Original language | English |
|---|---|
| Pages (from-to) | 750-759 |
| Number of pages | 10 |
| Journal | Reproduction, Fertility and Development |
| Volume | 20 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - Sep 23 2008 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Reproductive Medicine
- Animal Science and Zoology
- Molecular Biology
- Genetics
- Endocrinology
- Developmental Biology
Cite this
Prostate expression of endothelins and their receptors in rat growth. / Wada, Yoshihiro; Takahashi, Wataru; Latifpour, Jamshid; Weiss, Robert M.; Matsumoto, Koichiro; Matsui, Kunihiko; Yamada, Gen; Imamura, Takahisa.
In: Reproduction, Fertility and Development, Vol. 20, No. 7, 23.09.2008, p. 750-759.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Prostate expression of endothelins and their receptors in rat growth
AU - Wada, Yoshihiro
AU - Takahashi, Wataru
AU - Latifpour, Jamshid
AU - Weiss, Robert M.
AU - Matsumoto, Koichiro
AU - Matsui, Kunihiko
AU - Yamada, Gen
AU - Imamura, Takahisa
PY - 2008/9/23
Y1 - 2008/9/23
N2 - Endothelins (ETs) have been shown to have mitogenic effects on prostate cells in vitro. To study a relationship between ETs and prostate growth, we investigated expression of ET-1 and ET-3 and their receptors ETA and ETB in the dorsolateral (DLP) and ventral (VP) prostate lobes of rats aged 3 weeks, 3 months, and 22 months. The weight ratio of each lobe to the body was not different at 3 weeks but increased more in the DLP than in the VP at 3 months; the ratio did not change at 22 months. The density of the major receptor ETA, measured by radio-labelled ET-1 binding to prostate membrane particulates, was highest at 3 weeks in both prostate lobes and decreased thereafter. mRNA expression levels of ETA, ETB, ET-1, and ET-3 in the DLP and ETA in the VP, as assessed by relative multiplex reverse transcription-polymerase chain reaction, were highest at 3 weeks, and no significant change was seen in mRNA expression of ET-converting enzyme-1. The highest expression of ETA and the ligands at 3 weeks, followed by dramatic growth at 3 months in the DLP, suggests a role of ETA-mediated signal transduction in prostate growth of rats and possibly of humans.
AB - Endothelins (ETs) have been shown to have mitogenic effects on prostate cells in vitro. To study a relationship between ETs and prostate growth, we investigated expression of ET-1 and ET-3 and their receptors ETA and ETB in the dorsolateral (DLP) and ventral (VP) prostate lobes of rats aged 3 weeks, 3 months, and 22 months. The weight ratio of each lobe to the body was not different at 3 weeks but increased more in the DLP than in the VP at 3 months; the ratio did not change at 22 months. The density of the major receptor ETA, measured by radio-labelled ET-1 binding to prostate membrane particulates, was highest at 3 weeks in both prostate lobes and decreased thereafter. mRNA expression levels of ETA, ETB, ET-1, and ET-3 in the DLP and ETA in the VP, as assessed by relative multiplex reverse transcription-polymerase chain reaction, were highest at 3 weeks, and no significant change was seen in mRNA expression of ET-converting enzyme-1. The highest expression of ETA and the ligands at 3 weeks, followed by dramatic growth at 3 months in the DLP, suggests a role of ETA-mediated signal transduction in prostate growth of rats and possibly of humans.
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U2 - 10.1071/RD08052
DO - 10.1071/RD08052
M3 - Article
C2 - 18842177
AN - SCOPUS:51949099011
VL - 20
SP - 750
EP - 759
JO - Reproduction, Fertility and Development
JF - Reproduction, Fertility and Development
SN - 1031-3613
IS - 7
ER -