Proteasome affects the expression of aryl hydrocarbon receptor-regulated proteins

Takumi Ishida, Masayo Kawakami, Hiroko Baba, Masahiro Yahata, Junpei Mutoh, Shuso Takeda, Hideaki Fujita, Yoshitaka Tanaka, Yuji Ishii, Hideyuki Yamada

Research output: Contribution to journalArticle

Abstract

The effect of proteasome inhibition with N-acetyl-leucyl-leucyl-norleucinal (ALLN) on the protein expression regulated by aryl hydrocarbon receptor (AhR) was studied in T47D breast tumor cells. The luciferase reporter gene assay using a construct which has the xenobiotic responsive element showed that the inducible expression of the reporter with AhR ligands was significantly reduced by co-treatment with ALLN. The same suppressive effect by ALLN was observed for ethoxyresorufin O-deethylase (EROD) activity induced by an AhR ligand, 3-methylcholanthrene (3MC). Despite the above effects, the induced expression of CYP1A1 and CYP1B1 mRNAs was unaffected by ALLN. While lactacystin, another proteasome inhibitor, exhibited the same effect as ALLN on EROD activity induced by 3MC, leupeptin, which is one of the cysteine protease inhibitors, had no such effect. Based on the evidence obtained, it appears that proteasome inhibition results in a reduction in the expression of AhR-regulated proteins.

Original languageEnglish
Pages (from-to)348-354
Number of pages7
JournalEnvironmental Toxicology and Pharmacology
Volume26
Issue number3
DOIs
Publication statusPublished - Nov 1 2008

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Aryl Hydrocarbon Receptors
Proteasome Endopeptidase Complex
Cytochrome P-450 CYP1A1
Methylcholanthrene
Proteins
Ligands
Cysteine Proteinase Inhibitors
Proteasome Inhibitors
Xenobiotics
Luciferases
Reporter Genes
Tumors
Assays
Genes
Cells
acetylleucyl-leucyl-norleucinal
Breast Neoplasms
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Toxicology
  • Pharmacology
  • Health, Toxicology and Mutagenesis

Cite this

Ishida, T., Kawakami, M., Baba, H., Yahata, M., Mutoh, J., Takeda, S., ... Yamada, H. (2008). Proteasome affects the expression of aryl hydrocarbon receptor-regulated proteins. Environmental Toxicology and Pharmacology, 26(3), 348-354. https://doi.org/10.1016/j.etap.2008.07.004

Proteasome affects the expression of aryl hydrocarbon receptor-regulated proteins. / Ishida, Takumi; Kawakami, Masayo; Baba, Hiroko; Yahata, Masahiro; Mutoh, Junpei; Takeda, Shuso; Fujita, Hideaki; Tanaka, Yoshitaka; Ishii, Yuji; Yamada, Hideyuki.

In: Environmental Toxicology and Pharmacology, Vol. 26, No. 3, 01.11.2008, p. 348-354.

Research output: Contribution to journalArticle

Ishida, T, Kawakami, M, Baba, H, Yahata, M, Mutoh, J, Takeda, S, Fujita, H, Tanaka, Y, Ishii, Y & Yamada, H 2008, 'Proteasome affects the expression of aryl hydrocarbon receptor-regulated proteins', Environmental Toxicology and Pharmacology, vol. 26, no. 3, pp. 348-354. https://doi.org/10.1016/j.etap.2008.07.004
Ishida, Takumi ; Kawakami, Masayo ; Baba, Hiroko ; Yahata, Masahiro ; Mutoh, Junpei ; Takeda, Shuso ; Fujita, Hideaki ; Tanaka, Yoshitaka ; Ishii, Yuji ; Yamada, Hideyuki. / Proteasome affects the expression of aryl hydrocarbon receptor-regulated proteins. In: Environmental Toxicology and Pharmacology. 2008 ; Vol. 26, No. 3. pp. 348-354.
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