Protection against Campylobacter jejuni Infection in Suckling Mice by Anti-Flagellar Antibody

Yuji Ueki, Akiko Umeda, Shuji Fujimoto, Kazunobu Amako, Masao Mitsuyama

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

We obtained two monoclonal antibodies of IgM class and IgA class of immunoglobulin prepared from mouse spleen cells immunized with crude flagellar preparation, and a polyclonal antibody raised against purified flagellin monomer of Campylobacter jejuni in a rabbit. The specificity of the reaction of these antibodies for flagellar filament was confirmed by Western blotting and by immunoelectron microscopy. These antibodies caused agglutination of the bacteria and inhibited the motility of the bacteria. When a strain of C. jejuni was treated with IgM class monoclonal antibody before being inoculated into suckling mice, it reduced colonization of the intestinal tract by this bacteria. Inhibition of the colonization by IgA class monoclonal antibody was less effective than that of IgM class, and the polyclonal antibody consisting mostly of IgG class immunoglobulin was without effect.

Original languageEnglish
Pages (from-to)1161-1171
Number of pages11
JournalMICROBIOLOGY and IMMUNOLOGY
Volume31
Issue number12
DOIs
Publication statusPublished - 1987

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Virology

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