Protection by buckwheat polyphenols of cell death induced by glutamate and kainate in cultured hippocampal neurons

Pu Fengling, Kenichi Mishima, Keiichi Irie, Nobuaki Egashira, Katsunori Iwasaki, Michihiro Fujiwara, Tomoaki Ikeda, Hajime Fujii, Kenichi Kosuna

Research output: Contribution to journalArticle

Abstract

In the present study, we examined the effect of buckwheat polyphenols (BWP) on cell death induced by glutamate and kainate in primary cultures of hippocampal neurons by assay of lactate dehydrogenase (LDH) released out of cells. BWP (100 μg/mL) could significantly prevent cell death induced by 100 μM, but not that induced by 300 μM of glutamate, whereas MK-801, a N-methyl-D-aspartate (NMDA) receptor antagonist, remarkably inhibited cell death induced by either concentration of glutamate. BWP (100 μg/ml) and 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo(F)quinoxaline (NBQX), an α-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA) receptor antagonist, showed a protective effect on cell death induced by kainate (1 and 3 mM). These results suggest that BWP exhibits the neuroprotective action by inhibiting the AMPA receptor activity.

Original languageEnglish
Pages (from-to)143-146
Number of pages4
JournalJournal of Traditional Medicines
Volume21
Issue number3
DOIs
Publication statusPublished - Jan 1 2004
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Drug Discovery
  • Complementary and alternative medicine

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