TY - JOUR
T1 - Protection by buckwheat polyphenols of cell death induced by glutamate and kainate in cultured hippocampal neurons
AU - Fengling, Pu
AU - Mishima, Kenichi
AU - Irie, Keiichi
AU - Egashira, Nobuaki
AU - Iwasaki, Katsunori
AU - Fujiwara, Michihiro
AU - Ikeda, Tomoaki
AU - Fujii, Hajime
AU - Kosuna, Kenichi
PY - 2004
Y1 - 2004
N2 - In the present study, we examined the effect of buckwheat polyphenols (BWP) on cell death induced by glutamate and kainate in primary cultures of hippocampal neurons by assay of lactate dehydrogenase (LDH) released out of cells. BWP (100 μg/mL) could significantly prevent cell death induced by 100 μM, but not that induced by 300 μM of glutamate, whereas MK-801, a N-methyl-D-aspartate (NMDA) receptor antagonist, remarkably inhibited cell death induced by either concentration of glutamate. BWP (100 μg/ml) and 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo(F)quinoxaline (NBQX), an α-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA) receptor antagonist, showed a protective effect on cell death induced by kainate (1 and 3 mM). These results suggest that BWP exhibits the neuroprotective action by inhibiting the AMPA receptor activity.
AB - In the present study, we examined the effect of buckwheat polyphenols (BWP) on cell death induced by glutamate and kainate in primary cultures of hippocampal neurons by assay of lactate dehydrogenase (LDH) released out of cells. BWP (100 μg/mL) could significantly prevent cell death induced by 100 μM, but not that induced by 300 μM of glutamate, whereas MK-801, a N-methyl-D-aspartate (NMDA) receptor antagonist, remarkably inhibited cell death induced by either concentration of glutamate. BWP (100 μg/ml) and 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo(F)quinoxaline (NBQX), an α-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA) receptor antagonist, showed a protective effect on cell death induced by kainate (1 and 3 mM). These results suggest that BWP exhibits the neuroprotective action by inhibiting the AMPA receptor activity.
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U2 - 10.11339/jtm.21.143
DO - 10.11339/jtm.21.143
M3 - Article
AN - SCOPUS:85009548030
SN - 1880-1447
VL - 21
SP - 143
EP - 146
JO - Journal of Traditional Medicines
JF - Journal of Traditional Medicines
IS - 3
ER -