Abstract
Objective Several chemokines play important roles in recruiting the monocyte/macrophage lineage into adipose tissues. We previously found CCL19 was highly expressed in adipocytes cocultured with macrophages stimulated by endotoxin. This study aimed to evaluate the role of CCL19-CCR7 axis on obesity and insulin resistance. Methods Serum CCL19 concentration was examined in obese model mice challenged by endotoxin. CCL19 receptor-null, Ccr7-/-, mice and wild-type mice fed a high-fat diet or normal diet were used to investigate the role of CCL19 signals on obesity-associated inflammation. Results CCL19 protein was elevated in the sera of obese model mice challenged by endotoxin. Ccr7-/- mice were protected from diet-induced obesity and insulin resistance. The adipose tissue and liver expression of inflammatory genes of Ccr7-/- mice was much lower than in diet-induced obese mice. Ccr7-/- mice were protected from fatty liver and dyslipidemia and exhibited increased thermogenesis on high-fat feeding. CCL19 attracts activated dendritic cells (DC). The expression of the DC markers, CD11b and 11c, was not observed in the adipose tissues of Ccr7-/- mice fed a high-fat diet, which might be closely associated with the protection of these mice from obesity. Conclusions The CCL19-CCR7 pathway associates with the development of high-fat-induced obesity and insulin resistance.
| Original language | English |
|---|---|
| Pages (from-to) | 1460-1471 |
| Number of pages | 12 |
| Journal | Obesity |
| Volume | 23 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - Jul 1 2015 |
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All Science Journal Classification (ASJC) codes
- Medicine (miscellaneous)
- Endocrinology, Diabetes and Metabolism
- Endocrinology
- Nutrition and Dietetics
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Protection from diet-induced obesity and insulin resistance in mice lacking CCL19-CCR7 signaling. / Sano, Tomomi; Iwashita, Misaki; Nagayasu, Shintaro; Yamashita, Akiko; Shinjo, Takanori; Hashikata, Atsushi; Asano, Tomoichiro; Kushiyama, Akifumi; Ishimaru, Naozumi; Takahama, Yousuke; Nishimura, Fusanori.
In: Obesity, Vol. 23, No. 7, 01.07.2015, p. 1460-1471.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Protection from diet-induced obesity and insulin resistance in mice lacking CCL19-CCR7 signaling
AU - Sano, Tomomi
AU - Iwashita, Misaki
AU - Nagayasu, Shintaro
AU - Yamashita, Akiko
AU - Shinjo, Takanori
AU - Hashikata, Atsushi
AU - Asano, Tomoichiro
AU - Kushiyama, Akifumi
AU - Ishimaru, Naozumi
AU - Takahama, Yousuke
AU - Nishimura, Fusanori
PY - 2015/7/1
Y1 - 2015/7/1
N2 - Objective Several chemokines play important roles in recruiting the monocyte/macrophage lineage into adipose tissues. We previously found CCL19 was highly expressed in adipocytes cocultured with macrophages stimulated by endotoxin. This study aimed to evaluate the role of CCL19-CCR7 axis on obesity and insulin resistance. Methods Serum CCL19 concentration was examined in obese model mice challenged by endotoxin. CCL19 receptor-null, Ccr7-/-, mice and wild-type mice fed a high-fat diet or normal diet were used to investigate the role of CCL19 signals on obesity-associated inflammation. Results CCL19 protein was elevated in the sera of obese model mice challenged by endotoxin. Ccr7-/- mice were protected from diet-induced obesity and insulin resistance. The adipose tissue and liver expression of inflammatory genes of Ccr7-/- mice was much lower than in diet-induced obese mice. Ccr7-/- mice were protected from fatty liver and dyslipidemia and exhibited increased thermogenesis on high-fat feeding. CCL19 attracts activated dendritic cells (DC). The expression of the DC markers, CD11b and 11c, was not observed in the adipose tissues of Ccr7-/- mice fed a high-fat diet, which might be closely associated with the protection of these mice from obesity. Conclusions The CCL19-CCR7 pathway associates with the development of high-fat-induced obesity and insulin resistance.
AB - Objective Several chemokines play important roles in recruiting the monocyte/macrophage lineage into adipose tissues. We previously found CCL19 was highly expressed in adipocytes cocultured with macrophages stimulated by endotoxin. This study aimed to evaluate the role of CCL19-CCR7 axis on obesity and insulin resistance. Methods Serum CCL19 concentration was examined in obese model mice challenged by endotoxin. CCL19 receptor-null, Ccr7-/-, mice and wild-type mice fed a high-fat diet or normal diet were used to investigate the role of CCL19 signals on obesity-associated inflammation. Results CCL19 protein was elevated in the sera of obese model mice challenged by endotoxin. Ccr7-/- mice were protected from diet-induced obesity and insulin resistance. The adipose tissue and liver expression of inflammatory genes of Ccr7-/- mice was much lower than in diet-induced obese mice. Ccr7-/- mice were protected from fatty liver and dyslipidemia and exhibited increased thermogenesis on high-fat feeding. CCL19 attracts activated dendritic cells (DC). The expression of the DC markers, CD11b and 11c, was not observed in the adipose tissues of Ccr7-/- mice fed a high-fat diet, which might be closely associated with the protection of these mice from obesity. Conclusions The CCL19-CCR7 pathway associates with the development of high-fat-induced obesity and insulin resistance.
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U2 - 10.1002/oby.21127
DO - 10.1002/oby.21127
M3 - Article
C2 - 26097021
AN - SCOPUS:84933178175
VL - 23
SP - 1460
EP - 1471
JO - Obesity
JF - Obesity
SN - 1930-7381
IS - 7
ER -