Protection of insulin receptor substrate-3 from staurosporine-induced apoptosis

Yasushi Kaburagi, Shinobu Satoh, Ritsuko Yamamoto-Honda, Yuzuru Ito, Yasuo Akanuma, Hisahiko Sekihara, Kazuki Yasuda, Takehiko Sasazuki, Takashi Kadowaki, Yoshio Yazaki

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In primary adipocytes, insulin receptor substrate (IRS)-1 and -3 are expressed in a comparable amount and play distinct roles in insulin signaling. To examine the roles of these IRS in apoptosis inhibition, we evaluated staurosporine-induced apoptosis in Chinese hamster ovary (CHO) cells overexpressing human insulin receptor and IRS-1 or IRS-3. Overexpression of both IRS protected CHO cells from staurosporine-induced apoptosis. Overexpressed IRS-3 as well as IRS-1 enhanced phosphoinositide (PI) 3-kinase activity in response to insulin and increased phosphorylation of protein kinase B (PKB) at S473 and phosphorylation of one of the members of the forkhead transcription factor FKHRL1 on T32 in both insulin-untreated and -treated states. Treatment of these cells with a PI 3-kinase inhibitor LY294002 suppressed apoptosis-inhibitory effects of IRS-1 and IRS-3 as well as the phosphorylation of PKB and FKHRL1. These results indicate that both IRS-1 and IRS-3 take part in apoptosis inhibition through the PI 3-kinase/PKB/forkhead cascade.

Original languageEnglish
Pages (from-to)371-377
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume300
Issue number2
DOIs
Publication statusPublished - Jan 10 2003
Externally publishedYes

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Insulin Receptor Substrate Proteins
Staurosporine
Apoptosis
Proto-Oncogene Proteins c-akt
Phosphorylation
1-Phosphatidylinositol 4-Kinase
Phosphatidylinositols
Insulin Receptor
Insulin
Cricetulus
Phosphotransferases
Ovary
Cells
Forkhead Transcription Factors
2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
Substrates
Adipocytes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Kaburagi, Y., Satoh, S., Yamamoto-Honda, R., Ito, Y., Akanuma, Y., Sekihara, H., ... Yazaki, Y. (2003). Protection of insulin receptor substrate-3 from staurosporine-induced apoptosis. Biochemical and Biophysical Research Communications, 300(2), 371-377. https://doi.org/10.1016/S0006-291X(02)02855-3

Protection of insulin receptor substrate-3 from staurosporine-induced apoptosis. / Kaburagi, Yasushi; Satoh, Shinobu; Yamamoto-Honda, Ritsuko; Ito, Yuzuru; Akanuma, Yasuo; Sekihara, Hisahiko; Yasuda, Kazuki; Sasazuki, Takehiko; Kadowaki, Takashi; Yazaki, Yoshio.

In: Biochemical and Biophysical Research Communications, Vol. 300, No. 2, 10.01.2003, p. 371-377.

Research output: Contribution to journalArticle

Kaburagi, Y, Satoh, S, Yamamoto-Honda, R, Ito, Y, Akanuma, Y, Sekihara, H, Yasuda, K, Sasazuki, T, Kadowaki, T & Yazaki, Y 2003, 'Protection of insulin receptor substrate-3 from staurosporine-induced apoptosis', Biochemical and Biophysical Research Communications, vol. 300, no. 2, pp. 371-377. https://doi.org/10.1016/S0006-291X(02)02855-3
Kaburagi, Yasushi ; Satoh, Shinobu ; Yamamoto-Honda, Ritsuko ; Ito, Yuzuru ; Akanuma, Yasuo ; Sekihara, Hisahiko ; Yasuda, Kazuki ; Sasazuki, Takehiko ; Kadowaki, Takashi ; Yazaki, Yoshio. / Protection of insulin receptor substrate-3 from staurosporine-induced apoptosis. In: Biochemical and Biophysical Research Communications. 2003 ; Vol. 300, No. 2. pp. 371-377.
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