TY - JOUR
T1 - Protection of polynucleotides against nuclease-mediated hydrolysis by complexation with schizophyllan
AU - Mizu, Masami
AU - Koumoto, Kazuya
AU - Kimura, Taro
AU - Sakurai, Kazuo
AU - Shinkai, Seiji
N1 - Funding Information:
This work is financially supported by “Organization and Function”, PRESTO, and SORST programs in Japan Science and Technology Corporation (JST).
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2004/7
Y1 - 2004/7
N2 - Schizophyllan is a β-(1→3)-D-glucan existing as a triple helix in water and as a single chain in dimethylsulfoxide (DMSO), respectively. As we already reported, when some homo-polynucleotide (for example, poly(dA) or poly(C)) is added to the schizophyllan/DMSO solution and subsequently DMSO is exchanged for water, the single chain of schizophyllan (s-SPG) forms a complex with the polynucleotide. The present work demonstrates that the polynucleotide bound in the complex is more stable to nuclease-mediated hydrolysis than the polynucleotide itself (i.e., naked polynucleotide), using high-performance liquid chromatography and ultraviolet absorbance technique. A kinetic study for the hydrolysis clarified that the simple Michaelis-Menten relation is held and the maximum velocity for the complex is one-sixth as small as that of the naked polynucleotide. This low hydrolysis rate for the complex suggests that s-SPG is applicable to a carrier for antisense oligonucleotides.
AB - Schizophyllan is a β-(1→3)-D-glucan existing as a triple helix in water and as a single chain in dimethylsulfoxide (DMSO), respectively. As we already reported, when some homo-polynucleotide (for example, poly(dA) or poly(C)) is added to the schizophyllan/DMSO solution and subsequently DMSO is exchanged for water, the single chain of schizophyllan (s-SPG) forms a complex with the polynucleotide. The present work demonstrates that the polynucleotide bound in the complex is more stable to nuclease-mediated hydrolysis than the polynucleotide itself (i.e., naked polynucleotide), using high-performance liquid chromatography and ultraviolet absorbance technique. A kinetic study for the hydrolysis clarified that the simple Michaelis-Menten relation is held and the maximum velocity for the complex is one-sixth as small as that of the naked polynucleotide. This low hydrolysis rate for the complex suggests that s-SPG is applicable to a carrier for antisense oligonucleotides.
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U2 - 10.1016/j.biomaterials.2003.09.079
DO - 10.1016/j.biomaterials.2003.09.079
M3 - Article
C2 - 14967545
AN - SCOPUS:1142309690
SN - 0142-9612
VL - 25
SP - 3109
EP - 3116
JO - Biomaterials
JF - Biomaterials
IS - 15
ER -