Protection of polynucleotides against nuclease-mediated hydrolysis by complexation with schizophyllan

Masami Mizu, Kazuya Koumoto, Taro Kimura, Kazuo Sakurai, Seiji Shinkai

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28 Citations (Scopus)

Abstract

Schizophyllan is a β-(1→3)-D-glucan existing as a triple helix in water and as a single chain in dimethylsulfoxide (DMSO), respectively. As we already reported, when some homo-polynucleotide (for example, poly(dA) or poly(C)) is added to the schizophyllan/DMSO solution and subsequently DMSO is exchanged for water, the single chain of schizophyllan (s-SPG) forms a complex with the polynucleotide. The present work demonstrates that the polynucleotide bound in the complex is more stable to nuclease-mediated hydrolysis than the polynucleotide itself (i.e., naked polynucleotide), using high-performance liquid chromatography and ultraviolet absorbance technique. A kinetic study for the hydrolysis clarified that the simple Michaelis-Menten relation is held and the maximum velocity for the complex is one-sixth as small as that of the naked polynucleotide. This low hydrolysis rate for the complex suggests that s-SPG is applicable to a carrier for antisense oligonucleotides.

Original languageEnglish
Pages (from-to)3109-3116
Number of pages8
JournalBiomaterials
Volume25
Issue number15
DOIs
Publication statusPublished - Jul 2004

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All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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