Protective effect of Toki-shakuyaku-san on amyloid β25-35- induced neuronal damage in cultured rat cortical neurons

Nobuaki Egashira, Katsunori Iwasaki, Yuki Akiyoshi, Yuki Takagaki, Izzettin Hatip-Al-Khatib, Kenichi Mishima, Kouji Kurauchi, Tomoaki Ikeda, Michihiro Fujiwara

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31 Citations (Scopus)


Amyloid β protein (Aβ) is the major component of senile plaques, the pathological hallmark of the neurodegeneration associated with Alzheimer's disease (AD). This study investigated the effect of Toki-shakuyaku-san (TSS), a traditional medicine, on Aβ25-35-induced neuronal death and lipid peroxidation assessed by measuring lactate dehydrogenase (LDH) and malondialdehyde (MDA), respectively. Aβ25-35 at 10 μM induced neuronal damage and increased the LDH and MDA. TSS at concentrations of 100 and 300 μg/mL significantly reduced the Aβ25-35-induced neuronal death and the lipid peroxidation. These results suggest that TSS has a protective effect against Aβ25-35-induced neuronal damage. TSS may be beneficial for the treatment of AD.

Original languageEnglish
Pages (from-to)450-453
Number of pages4
JournalPhytotherapy Research
Issue number5
Publication statusPublished - May 1 2005
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Pharmacology

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