Abstract
Gabexate mesilate (GM), a serine protease inhibitor, often causes severe vascular injury. We previously reported that GM induced necrotic cell death via injury of the cell membrane in porcine aorta endothelial cells (PAECs). In the present study, we investigated the protective effects of amino acids against this GM-induced cell injury in PAECs. L-Cysteine (Cys), glycine (Gly), L-serine, L-glutamine (Gln), L-glutamate (Glu), L-proline, L-methionine, L-threonine, and L-isoleucine significantly inhibited the GM-induced decrease of cell viability. Gly showed the most potent effect among these amino acids. Gly, L-Cys, L-Glu, and L-Gln also inhibited the GM-induced increase in the number of necrotic cells stained by propidium iodide (PI). However, these amino acids had no effect on the GM-induced inhibition of trypsin activity. Strychnine, MK-801, or dichlorokynurenic acid did not affect the protective effect of Gly. Gly completely suppressed the GM-induced increase in PI uptake, which occurred immediately after exposure to GM. These findings suggest that Gly exerts protection against GM-induced cellular membrane injury, and several amino acids such as Gly may be useful for prophylaxis of the GM-induced severe vascular injury.
| Original language | English |
|---|---|
| Pages (from-to) | 238-245 |
| Number of pages | 8 |
| Journal | Journal of Pharmacological Sciences |
| Volume | 107 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Jul 28 2008 |
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All Science Journal Classification (ASJC) codes
- Molecular Medicine
- Pharmacology
Cite this
Protective effects of amino acids against Gabexate mesilate-induced cell injury in porcine-aorta endothelial cells. / Aki, Tomoko; Egashira, Nobuaki; Yamauchi, Yui; Hama, Mika; Yano, Takahisa; Itoh, Yoshinori; Yamada, Takaaki; Oishi, Ryozo.
In: Journal of Pharmacological Sciences, Vol. 107, No. 3, 28.07.2008, p. 238-245.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Protective effects of amino acids against Gabexate mesilate-induced cell injury in porcine-aorta endothelial cells
AU - Aki, Tomoko
AU - Egashira, Nobuaki
AU - Yamauchi, Yui
AU - Hama, Mika
AU - Yano, Takahisa
AU - Itoh, Yoshinori
AU - Yamada, Takaaki
AU - Oishi, Ryozo
PY - 2008/7/28
Y1 - 2008/7/28
N2 - Gabexate mesilate (GM), a serine protease inhibitor, often causes severe vascular injury. We previously reported that GM induced necrotic cell death via injury of the cell membrane in porcine aorta endothelial cells (PAECs). In the present study, we investigated the protective effects of amino acids against this GM-induced cell injury in PAECs. L-Cysteine (Cys), glycine (Gly), L-serine, L-glutamine (Gln), L-glutamate (Glu), L-proline, L-methionine, L-threonine, and L-isoleucine significantly inhibited the GM-induced decrease of cell viability. Gly showed the most potent effect among these amino acids. Gly, L-Cys, L-Glu, and L-Gln also inhibited the GM-induced increase in the number of necrotic cells stained by propidium iodide (PI). However, these amino acids had no effect on the GM-induced inhibition of trypsin activity. Strychnine, MK-801, or dichlorokynurenic acid did not affect the protective effect of Gly. Gly completely suppressed the GM-induced increase in PI uptake, which occurred immediately after exposure to GM. These findings suggest that Gly exerts protection against GM-induced cellular membrane injury, and several amino acids such as Gly may be useful for prophylaxis of the GM-induced severe vascular injury.
AB - Gabexate mesilate (GM), a serine protease inhibitor, often causes severe vascular injury. We previously reported that GM induced necrotic cell death via injury of the cell membrane in porcine aorta endothelial cells (PAECs). In the present study, we investigated the protective effects of amino acids against this GM-induced cell injury in PAECs. L-Cysteine (Cys), glycine (Gly), L-serine, L-glutamine (Gln), L-glutamate (Glu), L-proline, L-methionine, L-threonine, and L-isoleucine significantly inhibited the GM-induced decrease of cell viability. Gly showed the most potent effect among these amino acids. Gly, L-Cys, L-Glu, and L-Gln also inhibited the GM-induced increase in the number of necrotic cells stained by propidium iodide (PI). However, these amino acids had no effect on the GM-induced inhibition of trypsin activity. Strychnine, MK-801, or dichlorokynurenic acid did not affect the protective effect of Gly. Gly completely suppressed the GM-induced increase in PI uptake, which occurred immediately after exposure to GM. These findings suggest that Gly exerts protection against GM-induced cellular membrane injury, and several amino acids such as Gly may be useful for prophylaxis of the GM-induced severe vascular injury.
UR - http://www.scopus.com/inward/record.url?scp=47749090927&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=47749090927&partnerID=8YFLogxK
U2 - 10.1254/jphs.08053FP
DO - 10.1254/jphs.08053FP
M3 - Article
C2 - 18587221
AN - SCOPUS:47749090927
VL - 107
SP - 238
EP - 245
JO - Journal of Pharmacological Sciences
JF - Journal of Pharmacological Sciences
SN - 1347-8613
IS - 3
ER -