Protective effects of amino acids against Gabexate mesilate-induced cell injury in porcine-aorta endothelial cells

Tomoko Aki, Nobuaki Egashira, Yui Yamauchi, Mika Hama, Takahisa Yano, Yoshinori Itoh, Takaaki Yamada, Ryozo Oishi

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Gabexate mesilate (GM), a serine protease inhibitor, often causes severe vascular injury. We previously reported that GM induced necrotic cell death via injury of the cell membrane in porcine aorta endothelial cells (PAECs). In the present study, we investigated the protective effects of amino acids against this GM-induced cell injury in PAECs. L-Cysteine (Cys), glycine (Gly), L-serine, L-glutamine (Gln), L-glutamate (Glu), L-proline, L-methionine, L-threonine, and L-isoleucine significantly inhibited the GM-induced decrease of cell viability. Gly showed the most potent effect among these amino acids. Gly, L-Cys, L-Glu, and L-Gln also inhibited the GM-induced increase in the number of necrotic cells stained by propidium iodide (PI). However, these amino acids had no effect on the GM-induced inhibition of trypsin activity. Strychnine, MK-801, or dichlorokynurenic acid did not affect the protective effect of Gly. Gly completely suppressed the GM-induced increase in PI uptake, which occurred immediately after exposure to GM. These findings suggest that Gly exerts protection against GM-induced cellular membrane injury, and several amino acids such as Gly may be useful for prophylaxis of the GM-induced severe vascular injury.

Original languageEnglish
Pages (from-to)238-245
Number of pages8
JournalJournal of Pharmacological Sciences
Volume107
Issue number3
DOIs
Publication statusPublished - 2008

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Pharmacology

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