Protective role of the HOG pathway against the growth defect caused by impaired biosynthesis of complex sphingolipids in yeast Saccharomyces cerevisiae

Yutaro Yamaguchi, Yuka Katsuki, Seiya Tanaka, Ryotaro Kawaguchi, Hiroto Denda, Takuma Ikeda, Kouichi Funato, Motohiro Tani

Research output: Contribution to journalArticle

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Abstract

Complex sphingolipids play critical roles in various cellular events in the yeast Saccharomyces cerevisiae. To identify genes that are related to the growth defect caused by disruption of complex sphingolipid biosynthesis, we screened for suppressor mutations and multicopy suppressor genes that confer resistance against repression of AUR1 encoding inositol phosphorylceramide synthase. From the results of this screening, we found that the activation of high-osmolarity glycerol (HOG) pathway is involved in suppression of growth defect caused by impaired biosynthesis of complex sphingolipids. Furthermore, it was found that transcriptional regulation via Msn2, Msn4 and Sko1 is involved in the suppressive effect of the HOG pathway. Lack of the HOG pathway did not enhance the reductions in complex sphingolipid levels or the increase in ceramide level caused by the AUR1 repression, implying that the suppressive effect of the HOG pathway on the growth defect is not attributed to restoration of impaired biosynthesis of complex sphingolipids. On the contrary, the HOG pathway and Msn2/4-mediated transcriptional activation was involved in suppression of aberrant reactive oxygen species accumulation caused by the AUR1 repression. These results indicated that the HOG pathway plays pivotal roles in maintaining cell growth under impaired biosynthesis of complex sphingolipids.

Original languageEnglish
Pages (from-to)363-386
Number of pages24
JournalMolecular Microbiology
Volume107
Issue number3
DOIs
Publication statusPublished - Feb 1 2018

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Sphingolipids
Osmolar Concentration
Glycerol
Saccharomyces cerevisiae
Yeasts
Growth
Genetic Suppression
Suppressor Genes
Ceramides
Inositol
Transcriptional Activation
Reactive Oxygen Species
Genes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

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Protective role of the HOG pathway against the growth defect caused by impaired biosynthesis of complex sphingolipids in yeast Saccharomyces cerevisiae. / Yamaguchi, Yutaro; Katsuki, Yuka; Tanaka, Seiya; Kawaguchi, Ryotaro; Denda, Hiroto; Ikeda, Takuma; Funato, Kouichi; Tani, Motohiro.

In: Molecular Microbiology, Vol. 107, No. 3, 01.02.2018, p. 363-386.

Research output: Contribution to journalArticle

Yamaguchi, Yutaro ; Katsuki, Yuka ; Tanaka, Seiya ; Kawaguchi, Ryotaro ; Denda, Hiroto ; Ikeda, Takuma ; Funato, Kouichi ; Tani, Motohiro. / Protective role of the HOG pathway against the growth defect caused by impaired biosynthesis of complex sphingolipids in yeast Saccharomyces cerevisiae. In: Molecular Microbiology. 2018 ; Vol. 107, No. 3. pp. 363-386.
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