Protective roles of T cells and interleukin-15 in Escherichia coli infection in mice

M. Takano, H. Nishimura, Y. Kimura, Y. Mokuno, J. Washizu, S. Itohara, Y. Nimura, Y. Yoshikai

    Research output: Contribution to journalArticle

    65 Citations (Scopus)

    Abstract

    The number of γδ T cells in the peritoneal cavity was increased after an intraperitoneal (i.p.) infection with Escherichia coil in lipopolysaccharide (LPS)-responsive C3H/HeN mice but not in LPS- hyporesponsive C3H/HeJ mice. The γδ T cells preferentially expressed invariant Vγ6 and Vδ1 chains and proliferated to produce a large amount of gamma interferon in the presence of LPS. Mice depleted of γδ T cells by T- cell receptor δ gene mutation showed impaired resistance against E. coil as assessed by bacterial growth. Macrophages from C3H/HeN mice infected with E. coli expressed higher levels of interleukin-15 (IL-15) mRNA than those from the infected C3H/HeJ mice. Administration of anti-IL-15 monoclonal antibody inhibited, albeit partially, the appearance of γδ T cells in C3H/HeN mice after E. coli infection and diminished the host defense against the infection. These results suggest that LPS-stimulated γδ T cells play an important role in the host defense against E. coil infection and that IL-15 may be partly involved in the protection via an increase in the γδ T cells.

    Original languageEnglish
    Pages (from-to)3270-3278
    Number of pages9
    JournalInfection and Immunity
    Volume66
    Issue number7
    Publication statusPublished - Jul 1 1998

    Fingerprint

    Escherichia coli Infections
    Interleukin-15
    Inbred C3H Mouse
    T-Lymphocytes
    Lipopolysaccharides
    Infection
    T-Cell Receptor Genes
    Escherichia
    Peritoneal Cavity
    Interferon-gamma
    Macrophages
    Monoclonal Antibodies
    Escherichia coli
    Messenger RNA
    Mutation
    Growth

    All Science Journal Classification (ASJC) codes

    • Parasitology
    • Microbiology
    • Immunology
    • Infectious Diseases

    Cite this

    Takano, M., Nishimura, H., Kimura, Y., Mokuno, Y., Washizu, J., Itohara, S., ... Yoshikai, Y. (1998). Protective roles of T cells and interleukin-15 in Escherichia coli infection in mice. Infection and Immunity, 66(7), 3270-3278.

    Protective roles of T cells and interleukin-15 in Escherichia coli infection in mice. / Takano, M.; Nishimura, H.; Kimura, Y.; Mokuno, Y.; Washizu, J.; Itohara, S.; Nimura, Y.; Yoshikai, Y.

    In: Infection and Immunity, Vol. 66, No. 7, 01.07.1998, p. 3270-3278.

    Research output: Contribution to journalArticle

    Takano, M, Nishimura, H, Kimura, Y, Mokuno, Y, Washizu, J, Itohara, S, Nimura, Y & Yoshikai, Y 1998, 'Protective roles of T cells and interleukin-15 in Escherichia coli infection in mice', Infection and Immunity, vol. 66, no. 7, pp. 3270-3278.
    Takano M, Nishimura H, Kimura Y, Mokuno Y, Washizu J, Itohara S et al. Protective roles of T cells and interleukin-15 in Escherichia coli infection in mice. Infection and Immunity. 1998 Jul 1;66(7):3270-3278.
    Takano, M. ; Nishimura, H. ; Kimura, Y. ; Mokuno, Y. ; Washizu, J. ; Itohara, S. ; Nimura, Y. ; Yoshikai, Y. / Protective roles of T cells and interleukin-15 in Escherichia coli infection in mice. In: Infection and Immunity. 1998 ; Vol. 66, No. 7. pp. 3270-3278.
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