Protein aggregates are transported to vacuoles by a macroautophagic mechanism in nutrient-starved plant cells

Kiminori Toyooka, Yuji Moriyasu, Yumi Goto, Masaki Takeuchi, Hiroo Fukuda, Ken Matsuoka

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

When a fusion protein of cytochrome b5 (Cyt b5) and the red fluorescent protein (RFP) are expressed in tobacco BY-2 cells, the expressed protein forms intracellular aggregates that emit red fluorescence. When such cells are grown to the stationary phase or incubated in nutrient limited medium, RFP fluorescence can be detected in the vacuolar lumen. We investigated this transport mechanism using a limited-nitrogen model. E-64 and 3-methyladenine, which inhibit autophagic processes, blocked the transport of the RFP signal to the vacuole. We next traced the autophagic process in tobacco cells using YFP fused with the tobacco Atg8 homologue (YFP-NtAtg8) and analyzed the contribution of autophagy to the vacuolar transport of the aggregates. Under limited-nitrogen conditions, the aggregates were degraded in preference to other organelles, and the autophagosomes colocalized with the aggregates at a higher frequency than with mitochondria. This is the first demonstration that selective macroautophagic degradation can occur in plant cells.

Original languageEnglish
Pages (from-to)96-106
Number of pages11
JournalAutophagy
Volume2
Issue number2
DOIs
Publication statusPublished - Jan 1 2006
Externally publishedYes

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Plant Cells
Vacuoles
Tobacco
Food
Nitrogen
Fluorescence
Cytochromes b5
Autophagy
Organelles
Mitochondria
Proteins
Protein Aggregates
red fluorescent protein

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Protein aggregates are transported to vacuoles by a macroautophagic mechanism in nutrient-starved plant cells. / Toyooka, Kiminori; Moriyasu, Yuji; Goto, Yumi; Takeuchi, Masaki; Fukuda, Hiroo; Matsuoka, Ken.

In: Autophagy, Vol. 2, No. 2, 01.01.2006, p. 96-106.

Research output: Contribution to journalArticle

Toyooka, Kiminori ; Moriyasu, Yuji ; Goto, Yumi ; Takeuchi, Masaki ; Fukuda, Hiroo ; Matsuoka, Ken. / Protein aggregates are transported to vacuoles by a macroautophagic mechanism in nutrient-starved plant cells. In: Autophagy. 2006 ; Vol. 2, No. 2. pp. 96-106.
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