TY - JOUR
T1 - Protein corona components of polyethylene glycol-conjugated organosilica nanoparticles modulates macrophage uptake
AU - Kim, Hyungjin
AU - Röth, Daniel
AU - Isoe, Yasuhiro
AU - Hayashi, Koichiro
AU - Mochizuki, Chihiro
AU - Kalkum, Markus
AU - Nakamura, Michihiro
N1 - Funding Information:
This research was supported in part by the JSPS Bilateral Programs (M.N.) from Ministry of Education, Culture, Sports, Science and Technology (MEXT) , Grant-in-Aid for Scientific Research (C) 16K01358 (M.N.), 21500409 (M.N.), 25350530 (M.N.), and Grant-in-Aid for Challenging Research (Exploratory) No19K22534 (M.N.) from Japan Society for the Promotion of Science .
Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2021/3
Y1 - 2021/3
N2 - Fluorescent organosilica nanoparticles (FNP) conjugated with polyethylene glycol (PEG) of variant molecular weight (2 K, 12 K, 20 K, and 30 K) were prepared to investigate their cellular uptake by murine-derived macrophages. In a medium with FBS, the cellular uptake of FNP-PEGs was decreased as compared to a medium without FBS, indicating that protein corona on FNP-PEGs reduced cellular uptake. Bovine serum albumin (BSA) and hemoglobin (Hb) were detected as the most abundant components on all FNP-PEGs. Pre-coating of FNP-PEGs with BSA and Hb reduced the macrophage uptake in a medium without FBS, suggesting that these components might strengthen the stealth function of PEGs. Furthermore, there was more reduction in uptake of BSA- and Hb-coated FNP-PEGs from a medium with FBS than without FBS. BSA and Hb could be the stealth enhancement protein of FNP-PEGs in vitro.
AB - Fluorescent organosilica nanoparticles (FNP) conjugated with polyethylene glycol (PEG) of variant molecular weight (2 K, 12 K, 20 K, and 30 K) were prepared to investigate their cellular uptake by murine-derived macrophages. In a medium with FBS, the cellular uptake of FNP-PEGs was decreased as compared to a medium without FBS, indicating that protein corona on FNP-PEGs reduced cellular uptake. Bovine serum albumin (BSA) and hemoglobin (Hb) were detected as the most abundant components on all FNP-PEGs. Pre-coating of FNP-PEGs with BSA and Hb reduced the macrophage uptake in a medium without FBS, suggesting that these components might strengthen the stealth function of PEGs. Furthermore, there was more reduction in uptake of BSA- and Hb-coated FNP-PEGs from a medium with FBS than without FBS. BSA and Hb could be the stealth enhancement protein of FNP-PEGs in vitro.
UR - http://www.scopus.com/inward/record.url?scp=85098597376&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85098597376&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfb.2020.111527
DO - 10.1016/j.colsurfb.2020.111527
M3 - Article
C2 - 33387796
AN - SCOPUS:85098597376
VL - 199
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
SN - 0927-7765
M1 - 111527
ER -