TY - JOUR
T1 - Protein corona formation on epigallocatechin gallate-Au nanoparticles suppressed tumor accumulation
AU - Wakayama, Chihiro
AU - Inubushi, Sachiko
AU - Kunihisa, Tomonari
AU - Mizumoto, Sachiko
AU - Baba, Motoi
AU - Tanino, Hirokazu
AU - Cho, Ik Sung
AU - Ooya, Tooru
N1 - Funding Information:
We thank Miss Moemi Matsuda (Kobe University) to help cell culture studies. This study was financially supported by the Grant Program for the Creation of Local Universities and Regional Industries by the Cabinet Office, Government of Japan (Kobe vision for the healthcare of tomorrow), Hyogo Science and Technology Association, Japan (No. 2079), and the JSPS KAKENHI (Grant Nos. JP22H04545and 22K16462). This work was supported in part by “Advanced Research Infrastructure for Materials and Nanotechnology in Japan (ARIM)” of the Ministry of Education, Culture, Sports, Science and Technology (MEXT).
Funding Information:
We thank Miss Moemi Matsuda (Kobe University) to help cell culture studies. This study was financially supported by the Grant Program for the Creation of Local Universities and Regional Industries by the Cabinet Office, Government of Japan (Kobe vision for the healthcare of tomorrow), Hyogo Science and Technology Association, Japan (No. 2079 ), and the JSPS KAKENHI (Grant Nos. JP22H04545 and 22K16462 ). This work was supported in part by "Advanced Research Infrastructure for Materials and Nanotechnology in Japan (ARIM)" of the Ministry of Education, Culture, Sports, Science and Technology (MEXT).
Publisher Copyright:
© 2023 The Authors
PY - 2023/4
Y1 - 2023/4
N2 - Metal nanoparticles (NPs), such as gold NPs (AuNPs), are particularly sensitive to X-rays, and thus specific accumulation of AuNPs in a tumor would allow radiotherapy with low energy X-rays and reduced side effects. AuNPs can be generated using HAuCl4 and the natural polyphenol epigallocatechin-3-gallate (EGCG) in the presence of citrate. Here, we generated EGCG-AuNPs in the presence of several additives and examined the accumulation of these NPs in mouse tumors following intravenous administration. EGCG-AuNPs 15 nm in diameter in the presence of sodium alginate accumulated more in tumors compared to 40-nm-diameter EGCG-AuNPs. Furthermore, the results of in vitro cellular uptake and serum protein absorption studies suggest that adsorption of 15–16 kDa serum proteins to EGCG-AuNPs suppresses accumulation in tumors. Thus, tendency to adsorb specific proteins on EGCG-AuNPs surface should be tailored for enhancing their accumulation in tumors.
AB - Metal nanoparticles (NPs), such as gold NPs (AuNPs), are particularly sensitive to X-rays, and thus specific accumulation of AuNPs in a tumor would allow radiotherapy with low energy X-rays and reduced side effects. AuNPs can be generated using HAuCl4 and the natural polyphenol epigallocatechin-3-gallate (EGCG) in the presence of citrate. Here, we generated EGCG-AuNPs in the presence of several additives and examined the accumulation of these NPs in mouse tumors following intravenous administration. EGCG-AuNPs 15 nm in diameter in the presence of sodium alginate accumulated more in tumors compared to 40-nm-diameter EGCG-AuNPs. Furthermore, the results of in vitro cellular uptake and serum protein absorption studies suggest that adsorption of 15–16 kDa serum proteins to EGCG-AuNPs suppresses accumulation in tumors. Thus, tendency to adsorb specific proteins on EGCG-AuNPs surface should be tailored for enhancing their accumulation in tumors.
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U2 - 10.1016/j.jciso.2023.100074
DO - 10.1016/j.jciso.2023.100074
M3 - Article
AN - SCOPUS:85145829779
SN - 2666-934X
VL - 9
JO - JCIS Open
JF - JCIS Open
M1 - 100074
ER -