Protein coverage on polymer nanolayers leading to mesenchymal stem cell patterning

Jungmok You, Akihito Yoshida, June Seok Heo, Han Soo Kim, Hyun Ok Kim, Kaoru Tamada, Eunkyoung Kim

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Interactions of gelatin and albumin with a photo-reactive diphenylamino-s-triazine bridged p-phenylene vinylene polymer (DTOPV) were examined by using surface plasmon resonance (SPR) spectroscopy to explore the effect of the polymer structure on protein coverage of DTOPV nanofilms. The SPR data revealed a significant increase of gelatin adsorption on UV-DTOPV nanofilms, while the adsorption of albumin was decreased by UV exposure in the time frame of the experiment. We also found that the selective adsorption of these proteins was highly dependent on the protein concentration; the highest selectivity of protein adsorption was obtained at the lowest concentration (3.5 μg ml -1), while no selective adsorption was confirmed at high concentrations (350 and 1000 μg ml -1). The selective attachment of mesenchymal stem cells (MSCs) was directly correlated with the selective adsorption of these proteins onto DTOPV nanofilms. The MSCs attachment onto UV-DTOPV films was promoted with only small mass coverage of gelatin, which led to MSC patterning onto the patterned DTOPV nanofilms successfully. The role of cell adhesion proteins that we found in this study will be a clue to elucidate the complex response of biomolecules on functional polymer nanolayers, and contribute to build up biocompatible surfaces on various advanced materials for the sake of cell engineering and medical implants. This journal is

Original languageEnglish
Pages (from-to)17625-17632
Number of pages8
JournalPhysical Chemistry Chemical Physics
Volume13
Issue number39
DOIs
Publication statusPublished - Oct 21 2011
Externally publishedYes

Fingerprint

stem cells
Stem cells
Polymers
proteins
Adsorption
adsorption
gelatins
polymers
Gelatin
Proteins
Surface plasmon resonance
albumins
surface plasmon resonance
attachment
Albumins
Cell engineering
Functional polymers
Triazines
Cell adhesion
Biomolecules

All Science Journal Classification (ASJC) codes

  • Physics and Astronomy(all)
  • Physical and Theoretical Chemistry

Cite this

Protein coverage on polymer nanolayers leading to mesenchymal stem cell patterning. / You, Jungmok; Yoshida, Akihito; Heo, June Seok; Kim, Han Soo; Kim, Hyun Ok; Tamada, Kaoru; Kim, Eunkyoung.

In: Physical Chemistry Chemical Physics, Vol. 13, No. 39, 21.10.2011, p. 17625-17632.

Research output: Contribution to journalArticle

You, Jungmok ; Yoshida, Akihito ; Heo, June Seok ; Kim, Han Soo ; Kim, Hyun Ok ; Tamada, Kaoru ; Kim, Eunkyoung. / Protein coverage on polymer nanolayers leading to mesenchymal stem cell patterning. In: Physical Chemistry Chemical Physics. 2011 ; Vol. 13, No. 39. pp. 17625-17632.
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