Protein Crystallization in Microdroplets with the Aid of Electrically Induced Microbubbles

Akiho Hirao, Naotomo Tottori, Maasa Yokomori, Miho Tagawa, Shigeo S. Sugano, Shinya Sakuma, Yoko Yamanishi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present a novel concept of microfluidic system that enable promoting the protein crystal nucleation, monitoring the protein crystallization state, and analysis the crystal structure in a sequential process. This proposed system comprised of four main functional parts: (i) the protein crystal nucleation using an electrically induced microbubble injector, (ii) droplets generation by microchannel, (iii) monitoring of a crystal morphology in droplets to optimize their incubation time, (iv) sorting of droplets containing produced crystals suitable for X-ray crystallography and analyzing the protein structure. As the first step of this research, in this study, we developed a critical functional part of the concept that enable control of the protein crystallization and could confirm the mean number of protein crystal in droplets, which was treated by electrically induced bubble, increased approximately 1.5 times as compared with that of protein crystal in untreated droplets.

Original languageEnglish
Title of host publication34th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2021
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages168-171
Number of pages4
ISBN (Electronic)9781665419123
DOIs
Publication statusPublished - Jan 25 2021
Event34th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2021 - Virtual, Gainesville, United States
Duration: Jan 25 2021Jan 29 2021

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Volume2021-January
ISSN (Print)1084-6999

Conference

Conference34th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2021
CountryUnited States
CityVirtual, Gainesville
Period1/25/211/29/21

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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