Protein engineering and the study of muscle contraction in Drosophila flight muscles

J. Sparrow, D. Drummond, M. Peckham, E. Hennessey, D. White

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We describe an experimental approach to the use of genetics to study muscle contraction in Drosophila melanogaster. Mutations induced by in vitro mutagenesis are inserted into the genome of flies using P-element mediated transformation, permitting the effects of the mutant genes to be studied in vivo in the indirect flight muscles (IFMs). Details of how mechanical experiments can be performed on skinned IFMs, despite their small size, are provided. The effects of two in vitro actin mutations, G368E and E316K, are described. The problems of performing biochemical and biophysical experiments on the IFMs and their myofibrillar proteins are described, together with indications as to how these may be overcome.

Original languageEnglish
Pages (from-to)73-78
Number of pages6
JournalJournal of Cell Science
Volume98
Issue numberSUPPL. 14
Publication statusPublished - May 14 1991
Externally publishedYes

Fingerprint

Protein Engineering
Muscle Contraction
Drosophila
Muscles
Mutation
Drosophila melanogaster
Mutagenesis
Diptera
Actins
Genome
Genes
Proteins
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Sparrow, J., Drummond, D., Peckham, M., Hennessey, E., & White, D. (1991). Protein engineering and the study of muscle contraction in Drosophila flight muscles. Journal of Cell Science, 98(SUPPL. 14), 73-78.

Protein engineering and the study of muscle contraction in Drosophila flight muscles. / Sparrow, J.; Drummond, D.; Peckham, M.; Hennessey, E.; White, D.

In: Journal of Cell Science, Vol. 98, No. SUPPL. 14, 14.05.1991, p. 73-78.

Research output: Contribution to journalArticle

Sparrow, J, Drummond, D, Peckham, M, Hennessey, E & White, D 1991, 'Protein engineering and the study of muscle contraction in Drosophila flight muscles', Journal of Cell Science, vol. 98, no. SUPPL. 14, pp. 73-78.
Sparrow J, Drummond D, Peckham M, Hennessey E, White D. Protein engineering and the study of muscle contraction in Drosophila flight muscles. Journal of Cell Science. 1991 May 14;98(SUPPL. 14):73-78.
Sparrow, J. ; Drummond, D. ; Peckham, M. ; Hennessey, E. ; White, D. / Protein engineering and the study of muscle contraction in Drosophila flight muscles. In: Journal of Cell Science. 1991 ; Vol. 98, No. SUPPL. 14. pp. 73-78.
@article{388c69bd0a4942a8b0a8548d05fcee9b,
title = "Protein engineering and the study of muscle contraction in Drosophila flight muscles",
abstract = "We describe an experimental approach to the use of genetics to study muscle contraction in Drosophila melanogaster. Mutations induced by in vitro mutagenesis are inserted into the genome of flies using P-element mediated transformation, permitting the effects of the mutant genes to be studied in vivo in the indirect flight muscles (IFMs). Details of how mechanical experiments can be performed on skinned IFMs, despite their small size, are provided. The effects of two in vitro actin mutations, G368E and E316K, are described. The problems of performing biochemical and biophysical experiments on the IFMs and their myofibrillar proteins are described, together with indications as to how these may be overcome.",
author = "J. Sparrow and D. Drummond and M. Peckham and E. Hennessey and D. White",
year = "1991",
month = "5",
day = "14",
language = "English",
volume = "98",
pages = "73--78",
journal = "Journal of Cell Science",
issn = "0021-9533",
publisher = "Company of Biologists Ltd",
number = "SUPPL. 14",

}

TY - JOUR

T1 - Protein engineering and the study of muscle contraction in Drosophila flight muscles

AU - Sparrow, J.

AU - Drummond, D.

AU - Peckham, M.

AU - Hennessey, E.

AU - White, D.

PY - 1991/5/14

Y1 - 1991/5/14

N2 - We describe an experimental approach to the use of genetics to study muscle contraction in Drosophila melanogaster. Mutations induced by in vitro mutagenesis are inserted into the genome of flies using P-element mediated transformation, permitting the effects of the mutant genes to be studied in vivo in the indirect flight muscles (IFMs). Details of how mechanical experiments can be performed on skinned IFMs, despite their small size, are provided. The effects of two in vitro actin mutations, G368E and E316K, are described. The problems of performing biochemical and biophysical experiments on the IFMs and their myofibrillar proteins are described, together with indications as to how these may be overcome.

AB - We describe an experimental approach to the use of genetics to study muscle contraction in Drosophila melanogaster. Mutations induced by in vitro mutagenesis are inserted into the genome of flies using P-element mediated transformation, permitting the effects of the mutant genes to be studied in vivo in the indirect flight muscles (IFMs). Details of how mechanical experiments can be performed on skinned IFMs, despite their small size, are provided. The effects of two in vitro actin mutations, G368E and E316K, are described. The problems of performing biochemical and biophysical experiments on the IFMs and their myofibrillar proteins are described, together with indications as to how these may be overcome.

UR - http://www.scopus.com/inward/record.url?scp=0025828355&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025828355&partnerID=8YFLogxK

M3 - Article

C2 - 1909335

AN - SCOPUS:0025828355

VL - 98

SP - 73

EP - 78

JO - Journal of Cell Science

JF - Journal of Cell Science

SN - 0021-9533

IS - SUPPL. 14

ER -