Protein extraction by calix[6]arene in an aliphatic organic solvent

Kojiro Shimojo, Junji Watanabe, Tatsuya Oshima, Masahiro Goto

    Research output: Contribution to journalArticlepeer-review

    17 Citations (Scopus)

    Abstract

    Protein extraction was performed by a calix[6]arene carboxylic acid derivative in isooctane. Although the calixarene has a drawback in that it is not solubilized in aliphatic organic solvents, addition of alcohols overcomes the problem. The effect of alcohols on the extraction of cytochrome c (Cyt-c) was investigated with calix[6]arene in isooctane. Normal octanol provided the best result for the Cyt-c extraction. The pH value in the aqueous phase was one of the key operational factors in the liquid-liquid extraction process. It was confirmed that the extracted proteins maintain their biological activity during the extraction operation.

    Original languageEnglish
    Pages (from-to)185-189
    Number of pages5
    JournalSolvent Extraction Research and Development
    Volume10
    Publication statusPublished - Dec 1 2003

    All Science Journal Classification (ASJC) codes

    • Chemistry(all)
    • Chemical Engineering(all)

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