Protein extraction by calix[6]arene in an aliphatic organic solvent

Kojiro Shimojo, Junji Watanabe, Tatsuya Oshima, Masahiro Goto

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Protein extraction was performed by a calix[6]arene carboxylic acid derivative in isooctane. Although the calixarene has a drawback in that it is not solubilized in aliphatic organic solvents, addition of alcohols overcomes the problem. The effect of alcohols on the extraction of cytochrome c (Cyt-c) was investigated with calix[6]arene in isooctane. Normal octanol provided the best result for the Cyt-c extraction. The pH value in the aqueous phase was one of the key operational factors in the liquid-liquid extraction process. It was confirmed that the extracted proteins maintain their biological activity during the extraction operation.

Original languageEnglish
Pages (from-to)185-189
Number of pages5
JournalSolvent Extraction Research and Development
Volume10
Publication statusPublished - Dec 1 2003

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Organic solvents
Proteins
Cytochromes c
Alcohols
Calixarenes
Octanols
Liquids
Carboxylic Acids
Bioactivity
Carboxylic acids
calix(6)arene
Derivatives
2,2,4-trimethylpentane

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Protein extraction by calix[6]arene in an aliphatic organic solvent. / Shimojo, Kojiro; Watanabe, Junji; Oshima, Tatsuya; Goto, Masahiro.

In: Solvent Extraction Research and Development, Vol. 10, 01.12.2003, p. 185-189.

Research output: Contribution to journalArticle

Shimojo, Kojiro ; Watanabe, Junji ; Oshima, Tatsuya ; Goto, Masahiro. / Protein extraction by calix[6]arene in an aliphatic organic solvent. In: Solvent Extraction Research and Development. 2003 ; Vol. 10. pp. 185-189.
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