Protein-Grafted Polymers Prepared Through a Site-Specific Conjugation by Microbial Transglutaminase for an Immunosorbent Assay

Rie Wakabayashi, Kensuke Yahiro, Kounosuke Hayashi, Masahiro Goto, Noriho Kamiya

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Protein-polymer conjugates have been developed in many fields. Most hybrids are composed of one protein attached to one or several polymer chains. The other form of hybrid involves the construction of multiple proteins on one polymer chain, thereby facilitating protein assemblies that provide multivalent effects. Unfortunately, synthetic methods for production of these types of hybrids are limited and challenging because precise control of the conjugation sites is needed. Herein, a novel synthetic polymer that can enzymatically assemble multiple proteins was developed. Polyacrylamide grafted with multiple microbial transglutaminase (MTG)-recognizable peptide derivatives was synthesized, and MTG-catalyzed site-specific conjugation of proteins with the polymer was achieved. The application for immunological biosensing was demonstrated using the assembly of a fusion protein composed of antibody-binding and enzyme moieties. This enzymatic method to synthesize a one-dimensional protein assembly on a synthetic polymer is versatile and can be expanded to a wide range of applications.

Original languageEnglish
Pages (from-to)422-430
Number of pages9
JournalBiomacromolecules
Volume18
Issue number2
DOIs
Publication statusPublished - Feb 13 2017

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Immunosorbents
Transglutaminases
Assays
Polymers
Proteins
Polyacrylates
Antibodies
Peptides
Fusion reactions
Enzymes
Derivatives

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Protein-Grafted Polymers Prepared Through a Site-Specific Conjugation by Microbial Transglutaminase for an Immunosorbent Assay. / Wakabayashi, Rie; Yahiro, Kensuke; Hayashi, Kounosuke; Goto, Masahiro; Kamiya, Noriho.

In: Biomacromolecules, Vol. 18, No. 2, 13.02.2017, p. 422-430.

Research output: Contribution to journalArticle

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