Protein-immobilized Electrode for Rapid and Convenient Sensing of Thyroid Hormone Receptor-ligand Interaction

Masaharu Murata, Kentaro Yano, Shinichiro Kuroki, Tatsuo Suzutani, Yoshiki Katayama

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The recombinant human thyroid hormone receptor (TR) was expressed as an in-frame fusion with ten consecutive histidine residues using a bacterial system; then the receptor was immobilized on an Au-electrode with Ni(II)-mediated chemisorption using the histidine tag and thiol-modified nitrilotriacetic acid. The receptor-modified electrode could rapidly detect ligand binding to hTR without any separation procedures, and showed a good response in a concentration-dependent manner. The sensitivity of this biosensor based on ligand-receptor interactions was comparable to those of conventional competitive ligand binding assays using radio-labeled ligands. Our results strongly suggest that our new biosensor can be applied to the identification of new ligands for hTR.

Original languageEnglish
Pages (from-to)1569-1573
Number of pages5
Journalanalytical sciences
Volume19
Issue number12
DOIs
Publication statusPublished - Dec 2003

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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